Immunomodulation by oligofructose and inulin

K. A. Kelly-Quagliana, Randal Buddington, J. Van Loo, P. D. Nelson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A study was designed to examine the purported immunomodulating properties of oligofructose and inulin. The immune status of mice fed a control diet was compared with mice fed three diets containing 2.5% oligofructose, 10% oligofructose, or 10% inulin. The speed and efficiency of cell-mediated responses were evaluated by measuring natural killer cell (NK) activity in spleenocytes and by quantifying phagocytosis by peritoneal lavage macrophages. Immune cell population composition was evaluated by cytology of blood and tissue suspensions, WBC counts, and FACS analysis. FACS was used to examine CD4/CD8 and T/B ratios of thymocytes and spleenocytes and to examine cell distribution in peritoneal lavage. Although WBC counts for all treatments were within normal ranges, mice fed the control diet had twice as many WBCs compared to the other groups (pi0.0005). NK activity was higher in mice that were fed 10% oligofructose (pi0.02) or inulin (pi0.0005), implying an increased percentage of NK cells in the population or an increased speed of response. CD4/CD8 and T/B ratios did not differ, ruling out a re-distribution of maturing cell populations as a mode of immunomodulation. The analyses of phagocytosis capabilities are continuing. Preliminary results suggest oligofructose and inulin up-regulate front-line cell-mediated responses. Challenge studies are being used to evaluate whether the above indices provide protection again tumor cell lines, carcinogens, or pathogens.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998
Externally publishedYes

Fingerprint

immunomodulation
Inulin
Immunomodulation
fructooligosaccharides
inulin
natural killer cells
Nutrition
Natural Killer Cells
Peritoneal Lavage
Cells
mice
phagocytosis
leukocyte count
cells
Diet
Phagocytosis
cell lines
diet
Cytology
thymocytes

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Kelly-Quagliana, K. A., Buddington, R., Van Loo, J., & Nelson, P. D. (1998). Immunomodulation by oligofructose and inulin. FASEB Journal, 12(5).

Immunomodulation by oligofructose and inulin. / Kelly-Quagliana, K. A.; Buddington, Randal; Van Loo, J.; Nelson, P. D.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Kelly-Quagliana, KA, Buddington, R, Van Loo, J & Nelson, PD 1998, 'Immunomodulation by oligofructose and inulin', FASEB Journal, vol. 12, no. 5.
Kelly-Quagliana KA, Buddington R, Van Loo J, Nelson PD. Immunomodulation by oligofructose and inulin. FASEB Journal. 1998 Mar 20;12(5).
Kelly-Quagliana, K. A. ; Buddington, Randal ; Van Loo, J. ; Nelson, P. D. / Immunomodulation by oligofructose and inulin. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
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