Impact of obesity with impaired glucose tolerance on retinal degeneration in a rat model of metabolic syndrome

Kishore Kumar Godisela, Singareddy Sreenivasa Reddy, Chekkilla Uday Kumar, Natarajan Saravanan, Paduru Yadagiri Reddy, Monica Jablonski, Radha Ayyagari, Geereddy Bhanuprakash Reddy

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Abstract

Purpose: Metabolic syndrome (MetS) is associated with several degenerative diseases, including retinal degeneration. Previously, we reported on progressive retinal degeneration in a spontaneous obese rat (WNIN/Ob) model. In this study, we investigated the additional effect of impaired glucose tolerance (IGT), an essential component of MetS, on retinal degeneration using the WNIN/GR-Ob rat model. Methods: The retinal morphology and ultrastructure of WNIN/GR-Ob and age-matched littermate lean rats were studied by microscopy and immunohistochemistry. The retinal transcriptome of WNIN/GR-Ob was compared with the respective lean controls and with the WNIN/Ob model using microarray analysis. Expression of selected retinal marker genes was studied via real-time PCR. Results: Progressive loss of photoreceptor cells was observed in WNIN/GR-Ob rats with an onset as early as 3 months. Similarly, thinning of the inner nuclear layer was observed from 6 months in these rats. Immunohistochemical analysis showed decreased levels of rhodopsin and postsynaptic density protein-95 (PSD-95) proteins and increased levels of glial fibrillary acidic protein (GFAP), vascular endothelial growth factor (VEGF), and calretinin in WNIN/GR-Ob rats compared with the age-matched lean controls, further supporting cellular stress/damage and retinal degeneration. The retinal transcriptome analysis indicated altered expression profiles in both the WNIN/GR-Ob and WNIN/Ob rat models compared to their respective lean controls; these pathways are associated with activation of pathways like cellular oxidative stress response, inflammation, apoptosis, and phototransduction, although the changes were more prominent in WNIN/GR-Ob than in WNIN/Ob animals. Conclusions: WNIN/GR-Ob rats with added glucose intolerance developed retinal degeneration similar to the parent line WNIN/Ob. The severity of retinal degeneration was greater in WNIN/GR-Ob rats compared to WNIN/Ob, suggesting a possible role for IGT in this model. Hence, the WNIN/GR-Ob model could be a valuable tool for investigating the impact of MetS on retinal degeneration pathology.

Original languageEnglish (US)
Pages (from-to)263-274
Number of pages12
JournalMolecular Vision
Volume23
StatePublished - Apr 14 2017

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Retinal Degeneration
Glucose Intolerance
Obesity
Light Signal Transduction
Calbindin 2
Photoreceptor Cells
Rhodopsin
Glial Fibrillary Acidic Protein
Gene Expression Profiling
Microarray Analysis
Transcriptome
Vascular Endothelial Growth Factor A
Real-Time Polymerase Chain Reaction
Microscopy
Oxidative Stress
Immunohistochemistry
Apoptosis
Pathology
Inflammation

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Godisela, K. K., Reddy, S. S., Kumar, C. U., Saravanan, N., Reddy, P. Y., Jablonski, M., ... Reddy, G. B. (2017). Impact of obesity with impaired glucose tolerance on retinal degeneration in a rat model of metabolic syndrome. Molecular Vision, 23, 263-274.

Impact of obesity with impaired glucose tolerance on retinal degeneration in a rat model of metabolic syndrome. / Godisela, Kishore Kumar; Reddy, Singareddy Sreenivasa; Kumar, Chekkilla Uday; Saravanan, Natarajan; Reddy, Paduru Yadagiri; Jablonski, Monica; Ayyagari, Radha; Reddy, Geereddy Bhanuprakash.

In: Molecular Vision, Vol. 23, 14.04.2017, p. 263-274.

Research output: Contribution to journalArticle

Godisela, KK, Reddy, SS, Kumar, CU, Saravanan, N, Reddy, PY, Jablonski, M, Ayyagari, R & Reddy, GB 2017, 'Impact of obesity with impaired glucose tolerance on retinal degeneration in a rat model of metabolic syndrome', Molecular Vision, vol. 23, pp. 263-274.
Godisela, Kishore Kumar ; Reddy, Singareddy Sreenivasa ; Kumar, Chekkilla Uday ; Saravanan, Natarajan ; Reddy, Paduru Yadagiri ; Jablonski, Monica ; Ayyagari, Radha ; Reddy, Geereddy Bhanuprakash. / Impact of obesity with impaired glucose tolerance on retinal degeneration in a rat model of metabolic syndrome. In: Molecular Vision. 2017 ; Vol. 23. pp. 263-274.
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AU - Saravanan, Natarajan

AU - Reddy, Paduru Yadagiri

AU - Jablonski, Monica

AU - Ayyagari, Radha

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