Impairment of primary cilia contributes to visceral adiposity of high fat diet-fed mice

Ni Qiu, Wei Jin Fang, Hong Sheng Li, Zhi Min He, Zhousheng Xiao, Yan Xiong

Research output: Contribution to journalArticle

Abstract

Deficiency of primary cilia formation by knockout kinesin family member 3A (Kif3a) in mature osteoblasts led to osteopenia and enhanced adipogenesis. Adipogenesis plays an important role in adipose tissue expansion by High-fat-diet (HFD) induced obesity. Whether primary cilia participate in high-fat-diet induced adiposity remains unclear. In this study, we found that the number and length of primary cilia and expression levels of KIF3A and intraflagellar transport 88 homolog (IFT88) mRNA and proteins reached peak on the day 3 of adipogenesis, followed by a decrease to reach low basal expression levels at day 9 when differentiated to lipid accumulating adipocytes in VAT-SVFs derived from lean mice. The number of primary cilia was reduced by shRNA and chemical methods, leading to elevated transcripts of Pparγ, Cebp-α, Srebp-1, and Fasn and protein levels of PPARγ and FASN. Similar to the proadipogenic effect by the inhibition of primary cilia formation in control VAT-SVFs, HFD caused severe reduction of primary cilia formation and enhancement of adipogenesis in VAT-SVFs cultures. Flow cytometry analysis revealed percentage of G2/M phase cells and the protein expression of Cyclin A2 and CDK2 increased in control VAT-SVFs by knockdown of primary cilia with shRNA or chemical methods and HFD induced obese VAT-SVFs. In conclusion, the expression of primary cilia was in reverse correlation with adipogenic differentiation. HFD caused severe defects of primary cilia in VAT-SVFs, leading to adipose tissue expansion by enhancement of adipogenesis through promoting cell cycle re-entry at the early stage of adipogenesis.

Original languageEnglish (US)
Pages (from-to)1313-1325
Number of pages13
JournalJournal of Cellular Biochemistry
Volume119
Issue number2
DOIs
StatePublished - Feb 1 2018

Fingerprint

Cilia
Adiposity
High Fat Diet
Nutrition
Adipogenesis
Fats
Small Interfering RNA
Cyclin A2
Tissue Expansion
Tissue
Kinesin
Peroxisome Proliferator-Activated Receptors
Proteins
Flow cytometry
Reentry
Osteoblasts
Adipose Tissue
Cells
Lipids
Metabolic Bone Diseases

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Impairment of primary cilia contributes to visceral adiposity of high fat diet-fed mice. / Qiu, Ni; Fang, Wei Jin; Li, Hong Sheng; He, Zhi Min; Xiao, Zhousheng; Xiong, Yan.

In: Journal of Cellular Biochemistry, Vol. 119, No. 2, 01.02.2018, p. 1313-1325.

Research output: Contribution to journalArticle

Qiu, Ni ; Fang, Wei Jin ; Li, Hong Sheng ; He, Zhi Min ; Xiao, Zhousheng ; Xiong, Yan. / Impairment of primary cilia contributes to visceral adiposity of high fat diet-fed mice. In: Journal of Cellular Biochemistry. 2018 ; Vol. 119, No. 2. pp. 1313-1325.
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