Importance of chloride for the correction of chronic metabolic alkalosis in the rat

B. M. Wall, G. V. Byrum, J. H. Galla, R. G. Luke

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Abstract

To determine whether chloride repletion without sodium could correct chronic chloride depletion metabolic alkalosis (CDA) in Sprague-Dawley rats without volume expansion and without increasing glomerular filtration rate (GFR), CDA was generatged by peritoneal dialysis (PD) against 0.15 M NaHCO3 and maintained for 7-10 days by a chloride-restricted diet supplemented with sodium and potassium salts. Control animals were dialyzed against Ringer bicarbonate. The maintenance period of chronic CDA, compared with control, was characterized by hypokalemic metabolic alkalosis (serum TCO2 31.9 ± 0.6 vs. 23.1 ± 0.5 meq/l, P < 0.05), volume contraction (plasma volume 3.76 ± 0.08 vs. 4.19 ± 0.22 ml/100 g body wt, P < 0.05), decreased GFR (838 ± 84 vs. 1045 ± 45 μl·min-1·100 g body wt-1, P < 0.05), increased plasma renin activity (PRA) (63 ± 13 vs. 12 ± 3 ng·ml-1·h-1, P < 0.05), but unchanged plasma aldosterone concentrations (PAC) (4.1 ± 1.0 vs. 3.4 ± 1.6 ng/dl, P = NS). Complete correction of chronic CDA was accomplished by 24 h of ingestion of choline chloride drink, and despite negative sodium balance, neutral potassium balance, continued bicarbonate ingestion, and persistent volume contraction (plasma volume 3.76 ± 0.08 vs. 3.73 ± 0.12 ml/100 g body wt pre- and postcorrection, P = NS), GFR remained decreased (659 ± 87 vs. 1,045 ± 45 μl·min-1·100 g body wt-1, P < 0.05), but PAC did not change (4.1 ± 1.0 vs. 6.1 ± 1.6 ng/dl, P = NS) after correction of CDA. In separate infusions of 0.08 M chloride with calcium, magnesium, potassium, and lithium in rats subjected to either functional or sham nephrectomy, correction of CDA began only in the sham group in association with significant bicarbonaturia. Thus, in this model of chronic CDA, complete correction of chronic CDA was effected by chloride repletion despite sustained volume contraction, a sustained decrease in GFR, no decrease in PAC, and neutral potassium balance, thus emphasizing the key role of chloride repletion per se in allowing renal correction of chronic CDA.

Original languageEnglish (US)
Pages (from-to)22/5
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume253
Issue number5
StatePublished - Dec 1 1987

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Alkalosis
Chlorides
Glomerular Filtration Rate
Potassium
Aldosterone
Plasma Volume
Sodium
Bicarbonates
Eating
Calcium Chloride
Peritoneal Dialysis
Choline
Nephrectomy
Lithium
Renin
Magnesium
Sprague Dawley Rats

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Importance of chloride for the correction of chronic metabolic alkalosis in the rat. / Wall, B. M.; Byrum, G. V.; Galla, J. H.; Luke, R. G.

In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology, Vol. 253, No. 5, 01.12.1987, p. 22/5.

Research output: Contribution to journalArticle

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