Impression cytology

Recent advances and applications in dry eye disease

Eli Lopin, Tatiana Deveney, Penny Asbell

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Impression cytology (IC) allows cells to be harvested from the ocular surface noninvasively. Superficial layers of the epithelium are removed by application of cellulose acetate filters or Biopore membranes, and the cells can be subsequently analyzed by various methods, depending on the objective of the investigation or pathology involved. IC techniques are easily learned, can be performed in an outpatient setting, and cause virtually no discomfort to the patient. IC facilitates the diagnosis of ocular surface disorders, including, among others, keratoconjunctivitis sicca, ocular surface squamous neoplasia, and ocular surface infections. During the past decade, IC has been used increasingly to assist in diagnosis of ocular surface disease, improve our understanding of the pathophysiology of ocular surface disease, and provide biomarkers to be used as outcome measures in clinical trials. Dry eye disease is one area in which IC has contributed to significant advances.

Original languageEnglish (US)
Pages (from-to)93-110
Number of pages18
JournalOcular Surface
Volume7
Issue number2
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

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Eye Diseases
Cell Biology
Keratoconjunctivitis Sicca
Eye Infections
Outpatients
Epithelium
Biomarkers
Cell Membrane
Outcome Assessment (Health Care)
Clinical Trials
Pathology
Neoplasms

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Impression cytology : Recent advances and applications in dry eye disease. / Lopin, Eli; Deveney, Tatiana; Asbell, Penny.

In: Ocular Surface, Vol. 7, No. 2, 01.01.2009, p. 93-110.

Research output: Contribution to journalArticle

Lopin, Eli ; Deveney, Tatiana ; Asbell, Penny. / Impression cytology : Recent advances and applications in dry eye disease. In: Ocular Surface. 2009 ; Vol. 7, No. 2. pp. 93-110.
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