Improved incubation conditions for the Sandoz cyclosporine radioimmunoassay kit

J. D. Tyler, S. D. Looney, Mitchell Goldman

Research output: Contribution to journalArticle

Abstract

Numerous variations in the RIA, which purport to improve the precision and reproducibility of the RIA, including specimen preparation, while blood v serum or plasma samples, hematocrit determinations and temperature dependent partitioning of Cs between plasma and blood cells have been reported. Nevertheless, there is a scarcity of information concerning the suitability of the incubation conditions for the patient specimen, 3H-Cs tracer and anti-Cs antibody during the RIA, Accordingly, we performed a series of experiments to examine the effect of several combinations of incubation time and temperature on the precision and reproducibility of the Sandoz RIA assay.

Original languageEnglish (US)
Pages (from-to)370-373
Number of pages4
JournalTransplantation Proceedings
Volume20
Issue number2 SUPPL. 2
StatePublished - Jan 1 1988

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Cyclosporine
Radioimmunoassay
Temperature
Plasma Cells
Hematocrit
Anti-Idiotypic Antibodies
Blood Cells
Serum

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation

Cite this

Improved incubation conditions for the Sandoz cyclosporine radioimmunoassay kit. / Tyler, J. D.; Looney, S. D.; Goldman, Mitchell.

In: Transplantation Proceedings, Vol. 20, No. 2 SUPPL. 2, 01.01.1988, p. 370-373.

Research output: Contribution to journalArticle

Tyler, J. D. ; Looney, S. D. ; Goldman, Mitchell. / Improved incubation conditions for the Sandoz cyclosporine radioimmunoassay kit. In: Transplantation Proceedings. 1988 ; Vol. 20, No. 2 SUPPL. 2. pp. 370-373.
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