Improvement of two-way active avoidance memory requires protein kinase A activation and brain-derived neurotrophic factor expression in the dorsal hippocampus

Subimal Datta, Donald F. Siwek, Max P. Huang

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15 Citations (Scopus)

Abstract

Previous studies have shown that two-way active avoidance (TWAA) memory processing involves a functional interaction between the pontine wave (P wave) generator and the CA3 region of the dorsal hippocampus (DH-CA3). The present e periments examined whether the interaction between P wave generator activity and the DHCA3 involves the intracellular protein kinase A (PKA) signaling system. In the first series of experiments, rats were subjected to a session of TWAA training followed immediately by bilateral microinjection of either the PKA activation inhibit r (KT-5720) or vehicle control into the DH-CA3 and tested for TWAA memory 24 h later. The results indicated that immediate KT-5720 infusion impaired improvement of TWAA performance. Additional experiments showed that KT-5720 infusion also blocked TWAA training-induced BDNF expression in the DH-CA3. Together, these findings suggest that the PKA activation and BDNF expression in the DH-CA3 is essential for the improvement of TWAA memory.

Original languageEnglish (US)
Pages (from-to)257-264
Number of pages8
JournalJournal of Molecular Neuroscience
Volume38
Issue number3
DOIs
StatePublished - Jul 1 2009

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Brain-Derived Neurotrophic Factor
Cyclic AMP-Dependent Protein Kinases
Hippocampus
Microinjections
KT 5720

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

Cite this

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abstract = "Previous studies have shown that two-way active avoidance (TWAA) memory processing involves a functional interaction between the pontine wave (P wave) generator and the CA3 region of the dorsal hippocampus (DH-CA3). The present e periments examined whether the interaction between P wave generator activity and the DHCA3 involves the intracellular protein kinase A (PKA) signaling system. In the first series of experiments, rats were subjected to a session of TWAA training followed immediately by bilateral microinjection of either the PKA activation inhibit r (KT-5720) or vehicle control into the DH-CA3 and tested for TWAA memory 24 h later. The results indicated that immediate KT-5720 infusion impaired improvement of TWAA performance. Additional experiments showed that KT-5720 infusion also blocked TWAA training-induced BDNF expression in the DH-CA3. Together, these findings suggest that the PKA activation and BDNF expression in the DH-CA3 is essential for the improvement of TWAA memory.",
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AU - Siwek, Donald F.

AU - Huang, Max P.

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