In ovo electroporations of HH stage 10 chicken embryos

Marissa C. Blank, Viktor Chizhikov, Kathleen J. Millen

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Large size and external development of the chicken embryo have long made it a valuable tool in the study of developmental biology. With the advent of molecular biological techniques, the chick has become a useful system in which to study gene regulation and function. By electroporating DNA or RNA constructs into the developing chicken embryo, genes can be expressed or knocked down in orderto analyze in vivo gene function. Similarly, reporter constructs can be used for fate mapping or to examine putative gene regulatory elements. Compared to similar experiments in mouse, chick electroporation has the advantages of being quick, easy and inexpensive. This video demonstrates first how to make a window in the eggshell to manipulate the embryo. Next, the embryo is visualized with a dilute solution of India ink injected below the embryo. A glass needle and pipette are used to inject DNA and Fast Green dye into the developing neural tube, then platinum electrodes are placed parallel to the embryo and short electrical pulses are administered with a pulse generator. Finally, the egg is sealed with tape and placed back into an incubator for further development. Additionally, the video shows proper egg storage and handling and discusses possible causes of embryo loss following electroporation.

Original languageEnglish (US)
Article numbere408
JournalJournal of Visualized Experiments
Issue number9
DOIs
StatePublished - Oct 1 2007

Fingerprint

Electroporation
Chickens
Embryonic Structures
Genes
DNA
Pulse generators
Ovum
Platinum
RNA
Ink
Gene expression
Needles
Tapes
Embryo Loss
Egg Shell
Coloring Agents
Dyes
Incubators
Developmental Biology
Neural Tube

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

In ovo electroporations of HH stage 10 chicken embryos. / Blank, Marissa C.; Chizhikov, Viktor; Millen, Kathleen J.

In: Journal of Visualized Experiments, No. 9, e408, 01.10.2007.

Research output: Contribution to journalArticle

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