In situ end-labeling of human testicular tissue demonstrates increased apoptosis in conditions of abnormal spermatogenesis

William W. Lin, Larry I. Lipshultz, Dolores J. Lamb, Edward Kim, Thomas M. Wheeler

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

Objective: To determine, using an in situ end-labeling technique, whether the frequency of apoptosis is increased in testis biopsy specimens that demonstrate abnormal spermatogenesis. Design: Immunohistochemical analysis was performed on archived paraffin-embedded testis biopsy specimens. Apoptotic indices, defined as the number of apoptotic bodies per the total number of cells or the number of Sertoli cells, were calculated after counting all the intratubular spermatogenic cells and Sertoli cells in 20 tubules. Setting:. Major academic male factor infertility clinic. Patient(s): Forty-eight testis biopsy specimens were obtained for routine clinical purposes from 38 men with azoospermia or severe oligozoospermia. Intervention(s): In situ end-labeling was performed on archived paraffin- embedded testis biopsy specimens using terminal deoxynucleotidyl transferase. Main Outcome Measure(s): Apoptotic indices. Result(s): Significantly increased apoptotic indices were observed in patients with spermatocyte arrest, spermatid arrest, and hypospermatogenesis compared with patients with normal spermatogenesis and the Sertoli cell-only pattern. Conclusion(s): In situ end-labeling of testis biopsy specimens from infertile men demonstrates increased apoptosis in maturation arrest and hypospermatogenesis states compared with normal spermatogenesis and the Sertoli cell-only pattern. This unique observation implicates a prominent role for this form of programmed cell death in the pathophysiology of maturation arrest and hypospermatogenesis states.

Original languageEnglish (US)
Pages (from-to)1065-1069
Number of pages5
JournalFertility and Sterility
Volume68
Issue number6
DOIs
StatePublished - Dec 1 1997

Fingerprint

Spermatogenesis
Oligospermia
Sertoli Cells
Testis
Apoptosis
Biopsy
Paraffin
Azoospermia
Spermatocytes
Spermatids
DNA Nucleotidylexotransferase
Male Infertility
Cell Death
Cell Count
Observation
Outcome Assessment (Health Care)

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

In situ end-labeling of human testicular tissue demonstrates increased apoptosis in conditions of abnormal spermatogenesis. / Lin, William W.; Lipshultz, Larry I.; Lamb, Dolores J.; Kim, Edward; Wheeler, Thomas M.

In: Fertility and Sterility, Vol. 68, No. 6, 01.12.1997, p. 1065-1069.

Research output: Contribution to journalArticle

Lin, William W. ; Lipshultz, Larry I. ; Lamb, Dolores J. ; Kim, Edward ; Wheeler, Thomas M. / In situ end-labeling of human testicular tissue demonstrates increased apoptosis in conditions of abnormal spermatogenesis. In: Fertility and Sterility. 1997 ; Vol. 68, No. 6. pp. 1065-1069.
@article{27df32651b674c9cb07c81f906cc2bce,
title = "In situ end-labeling of human testicular tissue demonstrates increased apoptosis in conditions of abnormal spermatogenesis",
abstract = "Objective: To determine, using an in situ end-labeling technique, whether the frequency of apoptosis is increased in testis biopsy specimens that demonstrate abnormal spermatogenesis. Design: Immunohistochemical analysis was performed on archived paraffin-embedded testis biopsy specimens. Apoptotic indices, defined as the number of apoptotic bodies per the total number of cells or the number of Sertoli cells, were calculated after counting all the intratubular spermatogenic cells and Sertoli cells in 20 tubules. Setting:. Major academic male factor infertility clinic. Patient(s): Forty-eight testis biopsy specimens were obtained for routine clinical purposes from 38 men with azoospermia or severe oligozoospermia. Intervention(s): In situ end-labeling was performed on archived paraffin- embedded testis biopsy specimens using terminal deoxynucleotidyl transferase. Main Outcome Measure(s): Apoptotic indices. Result(s): Significantly increased apoptotic indices were observed in patients with spermatocyte arrest, spermatid arrest, and hypospermatogenesis compared with patients with normal spermatogenesis and the Sertoli cell-only pattern. Conclusion(s): In situ end-labeling of testis biopsy specimens from infertile men demonstrates increased apoptosis in maturation arrest and hypospermatogenesis states compared with normal spermatogenesis and the Sertoli cell-only pattern. This unique observation implicates a prominent role for this form of programmed cell death in the pathophysiology of maturation arrest and hypospermatogenesis states.",
author = "Lin, {William W.} and Lipshultz, {Larry I.} and Lamb, {Dolores J.} and Edward Kim and Wheeler, {Thomas M.}",
year = "1997",
month = "12",
day = "1",
doi = "10.1016/S0015-0282(97)00372-5",
language = "English (US)",
volume = "68",
pages = "1065--1069",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - In situ end-labeling of human testicular tissue demonstrates increased apoptosis in conditions of abnormal spermatogenesis

AU - Lin, William W.

AU - Lipshultz, Larry I.

AU - Lamb, Dolores J.

AU - Kim, Edward

AU - Wheeler, Thomas M.

PY - 1997/12/1

Y1 - 1997/12/1

N2 - Objective: To determine, using an in situ end-labeling technique, whether the frequency of apoptosis is increased in testis biopsy specimens that demonstrate abnormal spermatogenesis. Design: Immunohistochemical analysis was performed on archived paraffin-embedded testis biopsy specimens. Apoptotic indices, defined as the number of apoptotic bodies per the total number of cells or the number of Sertoli cells, were calculated after counting all the intratubular spermatogenic cells and Sertoli cells in 20 tubules. Setting:. Major academic male factor infertility clinic. Patient(s): Forty-eight testis biopsy specimens were obtained for routine clinical purposes from 38 men with azoospermia or severe oligozoospermia. Intervention(s): In situ end-labeling was performed on archived paraffin- embedded testis biopsy specimens using terminal deoxynucleotidyl transferase. Main Outcome Measure(s): Apoptotic indices. Result(s): Significantly increased apoptotic indices were observed in patients with spermatocyte arrest, spermatid arrest, and hypospermatogenesis compared with patients with normal spermatogenesis and the Sertoli cell-only pattern. Conclusion(s): In situ end-labeling of testis biopsy specimens from infertile men demonstrates increased apoptosis in maturation arrest and hypospermatogenesis states compared with normal spermatogenesis and the Sertoli cell-only pattern. This unique observation implicates a prominent role for this form of programmed cell death in the pathophysiology of maturation arrest and hypospermatogenesis states.

AB - Objective: To determine, using an in situ end-labeling technique, whether the frequency of apoptosis is increased in testis biopsy specimens that demonstrate abnormal spermatogenesis. Design: Immunohistochemical analysis was performed on archived paraffin-embedded testis biopsy specimens. Apoptotic indices, defined as the number of apoptotic bodies per the total number of cells or the number of Sertoli cells, were calculated after counting all the intratubular spermatogenic cells and Sertoli cells in 20 tubules. Setting:. Major academic male factor infertility clinic. Patient(s): Forty-eight testis biopsy specimens were obtained for routine clinical purposes from 38 men with azoospermia or severe oligozoospermia. Intervention(s): In situ end-labeling was performed on archived paraffin- embedded testis biopsy specimens using terminal deoxynucleotidyl transferase. Main Outcome Measure(s): Apoptotic indices. Result(s): Significantly increased apoptotic indices were observed in patients with spermatocyte arrest, spermatid arrest, and hypospermatogenesis compared with patients with normal spermatogenesis and the Sertoli cell-only pattern. Conclusion(s): In situ end-labeling of testis biopsy specimens from infertile men demonstrates increased apoptosis in maturation arrest and hypospermatogenesis states compared with normal spermatogenesis and the Sertoli cell-only pattern. This unique observation implicates a prominent role for this form of programmed cell death in the pathophysiology of maturation arrest and hypospermatogenesis states.

UR - http://www.scopus.com/inward/record.url?scp=0031450241&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031450241&partnerID=8YFLogxK

U2 - 10.1016/S0015-0282(97)00372-5

DO - 10.1016/S0015-0282(97)00372-5

M3 - Article

VL - 68

SP - 1065

EP - 1069

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 6

ER -