In vitro and in vivo evaluations of a novel post-electrospinning treatment to improve the fibrous structure of chitosan membranes for guided bone regeneration

Hengjie Su, Kwei Yu Liu, Anastasios Karydis, Daniel G. Abebe, Chaoxi Wu, Kenneth Anderson, Najib Ghadri, Pradeep Adatrow, Tomoko Fujiwara, Joel D. Bumgardner

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Electrospun chitosan membranes have been investigated for guided bone regeneration but are susceptible to swelling, dissolution, and loss of biomimetic nanofiber structure due to residual acid salts. A novel process was investigated for acidic salt removal from chitosan electrospun in 70% trifluoroacetic acid (TFA) by treating with triethylamine (TEA)/acetone and di-tert-butyl dicarbonate (tBOC) instead of the common Na2CO3 treatment. TFA salt removal and nanofiber structure stabilization were confirmed by EDS, FTIR, 19F NMR and electron microscopy before and after soaking in water. Membrane degradation after 4 weeks in PBS with 100g ml-1 lysozyme and osteoblastic proliferation were similar between TEA/tBOC-treated and Na2CO3-treated membranes. A simulated surgical tear test using surgical tacks showed that the peak tensile tear strength of the TEA/tBOC-treated chitosan membranes (62.1 ± 1.9 N mm-1) was significantly greater than a commercial polylactic acid (PLA) membrane (13.4 ± 0.4 N mm-1), similar to one commercial collagen membrane (55.3 ± 7.5 N mm-1) but lower than another commercial collagen membrane (133.9 ± 21.5 N mm-1). Rat 8 mm critical-sized calvarial defects covered with TEA/tBOC-treated chitosan membranes prevented soft tissue infiltration and supported new bone growth (15.76 ± 10.28%) similar to a commercial collagen membrane (16.08 ± 10.69%) at 12 weeks based on microCT analyses. Hence our novel TEA/tBOC process significantly improved nanofiber structure and mechanical strengths of electrospun chitosan membranes as compared to Na2CO3 treated membranes, without affecting in vitro degradation or cytocompatibility, improved membrane mechanical properties to be greater than a commercial PLA membrane and to be in range of commercial collagen membranes and supported calvarial bone defect healing similar to collagen. Thus TEA/tBOC-treated chitosan membranes exhibit many characteristics and properties that strongly support their potential for use in guided bone regeneration.

Original languageEnglish (US)
Article number015003
JournalBiomedical Materials (Bristol)
Volume12
Issue number1
DOIs
StatePublished - Feb 1 2017

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Chitosan
Electrospinning
Bone
Membranes
Collagen
Nanofibers
Trifluoroacetic acid
Salt removal
Trifluoroacetic Acid
Acids
Degradation
Defects
Biomimetics
Muramidase
Acetone
Infiltration
Electron microscopy
Strength of materials
Swelling
triethylamine

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

In vitro and in vivo evaluations of a novel post-electrospinning treatment to improve the fibrous structure of chitosan membranes for guided bone regeneration. / Su, Hengjie; Liu, Kwei Yu; Karydis, Anastasios; Abebe, Daniel G.; Wu, Chaoxi; Anderson, Kenneth; Ghadri, Najib; Adatrow, Pradeep; Fujiwara, Tomoko; Bumgardner, Joel D.

In: Biomedical Materials (Bristol), Vol. 12, No. 1, 015003, 01.02.2017.

Research output: Contribution to journalArticle

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AU - Karydis, Anastasios

AU - Abebe, Daniel G.

AU - Wu, Chaoxi

AU - Anderson, Kenneth

AU - Ghadri, Najib

AU - Adatrow, Pradeep

AU - Fujiwara, Tomoko

AU - Bumgardner, Joel D.

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