In vitro anti-bacterial and biological properties of magnetron co-sputtered silver-containing hydroxyapatite coating

W. Chen, Y. Liu, Harry Courtney, M. Bettenga, C. M. Agrawal, J. D. Bumgardner, J. L. Ong

Research output: Contribution to journalArticle

402 Citations (Scopus)

Abstract

Bacterial infection after implant placement is a significant rising complication. In order to reduce the incidence of implant-associated infections, several biomaterial surface treatments have been proposed. In this study, the effect of in vitro antibacterial activity and in vitro cytotoxicity of co-sputtered silver (Ag)-containing hydroxyapatite (HA) coating was evaluated. Deposition was achieved by a concurrent supply of 10 W to the Ag target and 300 W to the HA target. Heat treatment at 400 °C for 4 h was performed after 3 h deposition. X-ray diffraction, contact angles measurements, and surface roughness were used to characterize the coating surfaces. The RP12 strain of Staphylococcus epidermidis (ATCC 35984) and the Cowan I strain of Staphylococcus aureus were used to evaluate the antibacterial activity of the Ag-HA coatings, whereas human embryonic palatal mesenchyme cells, an osteoblast precursor cell line, were used to evaluate the in vitro cytotoxicity of the coatings. X-ray diffraction analysis performed in this study indicated peaks corresponding to Ag and HA on the co-sputtered Ag-HA surfaces. The contact angles for HA and Ag-HA surfaces were observed to be significantly lower when compared to Ti surfaces, whereas no significant difference in surface roughness was observed for all groups. In vitro bacterial adhesion study indicated a significantly reduced number of S. epidermidis and S. aureus on Ag-HA surface when compared to titanium (Ti) and HA surfaces. In addition, no significant difference in the in vitro cytotoxicty was observed between HA and Ag-HA surfaces. Overall, it was concluded that the creation of a multifunctional surface can be achieved by co-sputtering the osteoconductive HA with antibacterial Ag.

Original languageEnglish (US)
Pages (from-to)5512-5517
Number of pages6
JournalBiomaterials
Volume27
Issue number32
DOIs
StatePublished - Nov 1 2006

Fingerprint

Durapatite
Hydroxyapatite
Silver
Coatings
Staphylococcus epidermidis
Cytotoxicity
Titanium
X-Ray Diffraction
Contact angle
Staphylococcus aureus
In Vitro Techniques
Surface roughness
Bacterial Adhesion
Osteoblasts
Biocompatible Materials
Mesoderm
Angle measurement
Bacterial Infections
Biomaterials
X ray diffraction analysis

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Chen, W., Liu, Y., Courtney, H., Bettenga, M., Agrawal, C. M., Bumgardner, J. D., & Ong, J. L. (2006). In vitro anti-bacterial and biological properties of magnetron co-sputtered silver-containing hydroxyapatite coating. Biomaterials, 27(32), 5512-5517. https://doi.org/10.1016/j.biomaterials.2006.07.003

In vitro anti-bacterial and biological properties of magnetron co-sputtered silver-containing hydroxyapatite coating. / Chen, W.; Liu, Y.; Courtney, Harry; Bettenga, M.; Agrawal, C. M.; Bumgardner, J. D.; Ong, J. L.

In: Biomaterials, Vol. 27, No. 32, 01.11.2006, p. 5512-5517.

Research output: Contribution to journalArticle

Chen, W, Liu, Y, Courtney, H, Bettenga, M, Agrawal, CM, Bumgardner, JD & Ong, JL 2006, 'In vitro anti-bacterial and biological properties of magnetron co-sputtered silver-containing hydroxyapatite coating', Biomaterials, vol. 27, no. 32, pp. 5512-5517. https://doi.org/10.1016/j.biomaterials.2006.07.003
Chen, W. ; Liu, Y. ; Courtney, Harry ; Bettenga, M. ; Agrawal, C. M. ; Bumgardner, J. D. ; Ong, J. L. / In vitro anti-bacterial and biological properties of magnetron co-sputtered silver-containing hydroxyapatite coating. In: Biomaterials. 2006 ; Vol. 27, No. 32. pp. 5512-5517.
@article{baa242f5b8454b3487e0bfc23f43908e,
title = "In vitro anti-bacterial and biological properties of magnetron co-sputtered silver-containing hydroxyapatite coating",
abstract = "Bacterial infection after implant placement is a significant rising complication. In order to reduce the incidence of implant-associated infections, several biomaterial surface treatments have been proposed. In this study, the effect of in vitro antibacterial activity and in vitro cytotoxicity of co-sputtered silver (Ag)-containing hydroxyapatite (HA) coating was evaluated. Deposition was achieved by a concurrent supply of 10 W to the Ag target and 300 W to the HA target. Heat treatment at 400 °C for 4 h was performed after 3 h deposition. X-ray diffraction, contact angles measurements, and surface roughness were used to characterize the coating surfaces. The RP12 strain of Staphylococcus epidermidis (ATCC 35984) and the Cowan I strain of Staphylococcus aureus were used to evaluate the antibacterial activity of the Ag-HA coatings, whereas human embryonic palatal mesenchyme cells, an osteoblast precursor cell line, were used to evaluate the in vitro cytotoxicity of the coatings. X-ray diffraction analysis performed in this study indicated peaks corresponding to Ag and HA on the co-sputtered Ag-HA surfaces. The contact angles for HA and Ag-HA surfaces were observed to be significantly lower when compared to Ti surfaces, whereas no significant difference in surface roughness was observed for all groups. In vitro bacterial adhesion study indicated a significantly reduced number of S. epidermidis and S. aureus on Ag-HA surface when compared to titanium (Ti) and HA surfaces. In addition, no significant difference in the in vitro cytotoxicty was observed between HA and Ag-HA surfaces. Overall, it was concluded that the creation of a multifunctional surface can be achieved by co-sputtering the osteoconductive HA with antibacterial Ag.",
author = "W. Chen and Y. Liu and Harry Courtney and M. Bettenga and Agrawal, {C. M.} and Bumgardner, {J. D.} and Ong, {J. L.}",
year = "2006",
month = "11",
day = "1",
doi = "10.1016/j.biomaterials.2006.07.003",
language = "English (US)",
volume = "27",
pages = "5512--5517",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "32",

}

TY - JOUR

T1 - In vitro anti-bacterial and biological properties of magnetron co-sputtered silver-containing hydroxyapatite coating

AU - Chen, W.

AU - Liu, Y.

AU - Courtney, Harry

AU - Bettenga, M.

AU - Agrawal, C. M.

AU - Bumgardner, J. D.

AU - Ong, J. L.

PY - 2006/11/1

Y1 - 2006/11/1

N2 - Bacterial infection after implant placement is a significant rising complication. In order to reduce the incidence of implant-associated infections, several biomaterial surface treatments have been proposed. In this study, the effect of in vitro antibacterial activity and in vitro cytotoxicity of co-sputtered silver (Ag)-containing hydroxyapatite (HA) coating was evaluated. Deposition was achieved by a concurrent supply of 10 W to the Ag target and 300 W to the HA target. Heat treatment at 400 °C for 4 h was performed after 3 h deposition. X-ray diffraction, contact angles measurements, and surface roughness were used to characterize the coating surfaces. The RP12 strain of Staphylococcus epidermidis (ATCC 35984) and the Cowan I strain of Staphylococcus aureus were used to evaluate the antibacterial activity of the Ag-HA coatings, whereas human embryonic palatal mesenchyme cells, an osteoblast precursor cell line, were used to evaluate the in vitro cytotoxicity of the coatings. X-ray diffraction analysis performed in this study indicated peaks corresponding to Ag and HA on the co-sputtered Ag-HA surfaces. The contact angles for HA and Ag-HA surfaces were observed to be significantly lower when compared to Ti surfaces, whereas no significant difference in surface roughness was observed for all groups. In vitro bacterial adhesion study indicated a significantly reduced number of S. epidermidis and S. aureus on Ag-HA surface when compared to titanium (Ti) and HA surfaces. In addition, no significant difference in the in vitro cytotoxicty was observed between HA and Ag-HA surfaces. Overall, it was concluded that the creation of a multifunctional surface can be achieved by co-sputtering the osteoconductive HA with antibacterial Ag.

AB - Bacterial infection after implant placement is a significant rising complication. In order to reduce the incidence of implant-associated infections, several biomaterial surface treatments have been proposed. In this study, the effect of in vitro antibacterial activity and in vitro cytotoxicity of co-sputtered silver (Ag)-containing hydroxyapatite (HA) coating was evaluated. Deposition was achieved by a concurrent supply of 10 W to the Ag target and 300 W to the HA target. Heat treatment at 400 °C for 4 h was performed after 3 h deposition. X-ray diffraction, contact angles measurements, and surface roughness were used to characterize the coating surfaces. The RP12 strain of Staphylococcus epidermidis (ATCC 35984) and the Cowan I strain of Staphylococcus aureus were used to evaluate the antibacterial activity of the Ag-HA coatings, whereas human embryonic palatal mesenchyme cells, an osteoblast precursor cell line, were used to evaluate the in vitro cytotoxicity of the coatings. X-ray diffraction analysis performed in this study indicated peaks corresponding to Ag and HA on the co-sputtered Ag-HA surfaces. The contact angles for HA and Ag-HA surfaces were observed to be significantly lower when compared to Ti surfaces, whereas no significant difference in surface roughness was observed for all groups. In vitro bacterial adhesion study indicated a significantly reduced number of S. epidermidis and S. aureus on Ag-HA surface when compared to titanium (Ti) and HA surfaces. In addition, no significant difference in the in vitro cytotoxicty was observed between HA and Ag-HA surfaces. Overall, it was concluded that the creation of a multifunctional surface can be achieved by co-sputtering the osteoconductive HA with antibacterial Ag.

UR - http://www.scopus.com/inward/record.url?scp=33746840674&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746840674&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2006.07.003

DO - 10.1016/j.biomaterials.2006.07.003

M3 - Article

VL - 27

SP - 5512

EP - 5517

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 32

ER -