In vitro characterization of human dental pulp cells

Various isolation methods and culturing environments

George Huang, Wataru Sonoyama, James Chen, Sang Hyuk Park

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Our purpose was to characterize human dental pulp cells isolated by various methods and to examine the behavior of cells grown under various conditions for the purpose of pulp/dentin tissue engineering and regeneration. We compared the growth of human pulp cells isolated by either enzyme digestion or the outgrowth method. Expression of dentin sialophosphoprotein, Cbfa1, and two types of collagen (I and III) in these cells was examined by Western blot or reverse transcription/polymerase chain reaction. Growth of pulp cells on dentin and in collagen gel was also characterized. We found that different isolation methods give rise to different populations or lineages of pulp cells during in vitro passage based on their collagen gene expression patterns. Cells isolated by enzymedigestion had a higher proliferation rate than those isolated by outgrowth. Pulp cells did not proliferate or grew minimally on chemically and mechanically treated dentin surface and appeared to establish an odontoblast-like morphology with a cytoplasmic process extending into a dentinal tubule as revealed by scanning electron microscopy. The contraction of the collagen matrix caused by pulp cells was dramatic: down to 34% on day 14. Our data indicate that (1) the choice of the pulp cell isolation method may affect the distribution of the obtained cell populations, (2) a treated dentin surface might still promote odontoblast differentiation, and (3) a collagen matrix may not be a suitable scaffold for pulp tissue regeneration because of the marked contraction caused by pulp cells in the matrix. The present study thus provides important information and a basis for further investigations pre-requisite to establishing pulp tissue engineering/regeneration protocols.

Original languageEnglish (US)
Pages (from-to)225-236
Number of pages12
JournalCell and Tissue Research
Volume324
Issue number2
DOIs
StatePublished - May 1 2006

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Dental Pulp
Cell Separation
Dentin
Collagen
Odontoblasts
Regeneration
Tissue Engineering
In Vitro Techniques
Tissue Scaffolds
Collagen Type III
Growth
Collagen Type I
Electron Scanning Microscopy
Population
Reverse Transcription
Digestion
Western Blotting
Gels

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

In vitro characterization of human dental pulp cells : Various isolation methods and culturing environments. / Huang, George; Sonoyama, Wataru; Chen, James; Park, Sang Hyuk.

In: Cell and Tissue Research, Vol. 324, No. 2, 01.05.2006, p. 225-236.

Research output: Contribution to journalArticle

Huang, George ; Sonoyama, Wataru ; Chen, James ; Park, Sang Hyuk. / In vitro characterization of human dental pulp cells : Various isolation methods and culturing environments. In: Cell and Tissue Research. 2006 ; Vol. 324, No. 2. pp. 225-236.
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