In vitro effect of S. Mutans biofilm on fluoride/MDPB-containing adhesive system bonded to caries-affected primary dentin

Fabíola Galbiatti De Carvalho, Julia Puppin-Rontani, Rogério Lacerda Dos Santos, Hugo Lemes Carlo, Paulo Rogério Ferreti Bonan, Franklin Garcia-Godoy, Regina Maria Puppin-Rontani

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose: To evaluate the effect of storage in S. mutans culture or water on the durability of fluoride and MDPB-containing adhesive system on artificial caries-affected primary dentin.

Methods: Dentin flat surfaces of primary molars were submitted to artificial caries development using S. mutans biofilm. CAPD cavities were restored with Adper Scotchbond Multi-Purpose (SBM) or Clearfil Protect Bond (CPB) and they were serially sectioned into sticks (1 mm2 cross-sectional area) and stored in S. mutans biofilm for 3 days, in deionized water for 3 months and afterwards subjected to microtensile bond strength (μTBS) test. A control group was submitted to immediate |iTBS testing. Two-way ANOVA and Tukey's tests were used. Failure sites were observed by SEM and classified as cohesive (dentin or resin) and adhesive failures.

Results: The control group showed the highest μTBS values (MPa) for both SBM (25.2±8.5) and CPB (15.6±6.1) adhesive systems. A significant decrease in jxTBS values after S. mutans biofilm and water storage was observed for SBM (18.7±5.7 and 17.4±4.1, respectively) and CPB (13.9±5.2 and 13.7±4.8, respectively), but no difference was found between them. The highest percentage of adhesive failure was observed for all groups. However, cohesive failure in dentin was observed in a higher percentage for SBM than CP.

Original languageEnglish (US)
Pages (from-to)227-232
Number of pages6
JournalAmerican journal of dentistry
Volume27
Issue number5
StatePublished - Oct 1 2014
Externally publishedYes

Fingerprint

Dentin
Biofilms
Fluorides
Adhesives
Water
Control Groups
Continuous Ambulatory Peritoneal Dialysis
Analysis of Variance
Scotchbond Multi-Purpose
In Vitro Techniques
Clearfil Protect Bond

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

De Carvalho, F. G., Puppin-Rontani, J., Dos Santos, R. L., Carlo, H. L., Bonan, P. R. F., Garcia-Godoy, F., & Puppin-Rontani, R. M. (2014). In vitro effect of S. Mutans biofilm on fluoride/MDPB-containing adhesive system bonded to caries-affected primary dentin. American journal of dentistry, 27(5), 227-232.

In vitro effect of S. Mutans biofilm on fluoride/MDPB-containing adhesive system bonded to caries-affected primary dentin. / De Carvalho, Fabíola Galbiatti; Puppin-Rontani, Julia; Dos Santos, Rogério Lacerda; Carlo, Hugo Lemes; Bonan, Paulo Rogério Ferreti; Garcia-Godoy, Franklin; Puppin-Rontani, Regina Maria.

In: American journal of dentistry, Vol. 27, No. 5, 01.10.2014, p. 227-232.

Research output: Contribution to journalArticle

De Carvalho, FG, Puppin-Rontani, J, Dos Santos, RL, Carlo, HL, Bonan, PRF, Garcia-Godoy, F & Puppin-Rontani, RM 2014, 'In vitro effect of S. Mutans biofilm on fluoride/MDPB-containing adhesive system bonded to caries-affected primary dentin', American journal of dentistry, vol. 27, no. 5, pp. 227-232.
De Carvalho, Fabíola Galbiatti ; Puppin-Rontani, Julia ; Dos Santos, Rogério Lacerda ; Carlo, Hugo Lemes ; Bonan, Paulo Rogério Ferreti ; Garcia-Godoy, Franklin ; Puppin-Rontani, Regina Maria. / In vitro effect of S. Mutans biofilm on fluoride/MDPB-containing adhesive system bonded to caries-affected primary dentin. In: American journal of dentistry. 2014 ; Vol. 27, No. 5. pp. 227-232.
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AU - De Carvalho, Fabíola Galbiatti

AU - Puppin-Rontani, Julia

AU - Dos Santos, Rogério Lacerda

AU - Carlo, Hugo Lemes

AU - Bonan, Paulo Rogério Ferreti

AU - Garcia-Godoy, Franklin

AU - Puppin-Rontani, Regina Maria

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