In vitro evaluation of antioxidant and cytotoxic activities of lignin fractions extracted from Acacia nilotica

Anand Barapatre, Avtar Meena, Sowmya Mekala, Amitava Das, Harit Jha

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Lignin is one of the most important phytomacromolecule with diverse therapeutic properties such as anticancer, antimicrobial, anti-inflammatory and immune-stimulatory. The present study was carried out to evaluate the in vitro antioxidant, free radical scavenging and anti-proliferative/cytotoxic activities of eleven different lignin fractions, extracted from the wood of Acacia nilotica by pressurized solvent extraction (PSE) and successive solvent extraction (SSE) methods. Results indicate that the PSE fractions have high polyphenolic content and reducing power. However, the antioxidant efficiency examined by DPPH and ABTS radical scavenging assay was higher in SSE fractions. All lignin fractions revealed a significant ability to scavenge nitric oxide, hydroxyl and superoxide radicals. The extracted lignin fractions display high ferric ion reducing capacity and also possess excellent antioxidant potential in the hydrophobic (linoleic acid) system. Fractions extracted by polar solvent has the highest iron (Fe 2+ ) chelating activity as compared to other factions, indicating their effect on the redox cycling of iron. Four lignin fractions depicted higher cytotoxic potential (IC 50 : 2-15 μg/mL) towards breast cancer cell line (MCF-7) but were ineffective (IC 50 : ≥100 μg/mL) against normal primary human hepatic stellate cells (HHSteCs). These findings suggest that the lignin extracts of A. nilotica wood has a remarkable potential to prevent disease caused by the overproduction of radicals and also seem to be a promising candidate as natural antioxidant and anti-cancer agents.

Original languageEnglish (US)
Pages (from-to)443-453
Number of pages11
JournalInternational Journal of Biological Macromolecules
Volume86
DOIs
StatePublished - May 1 2016

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Acacia
Lignin
Antioxidants
Solvent extraction
Scavenging
Wood
Iron
Linoleic acid
Hepatic Stellate Cells
Nitric oxide
Linoleic Acid
Chelation
Free radicals
Superoxides
Hydroxyl Radical
Oxidation-Reduction
Free Radicals
In Vitro Techniques
Evaluation
Assays

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

In vitro evaluation of antioxidant and cytotoxic activities of lignin fractions extracted from Acacia nilotica. / Barapatre, Anand; Meena, Avtar; Mekala, Sowmya; Das, Amitava; Jha, Harit.

In: International Journal of Biological Macromolecules, Vol. 86, 01.05.2016, p. 443-453.

Research output: Contribution to journalArticle

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