In vitro inhibition of growth and induction of apoptosis in cancer cell lines by thymoquinone.

Ahmed M. Shoieb, Mona Elgayyar, Paul S. Dudrick, John Bell, Patricia K. Tithof

Research output: Contribution to journalArticle

172 Citations (Scopus)

Abstract

Thymoquinone (TQ) is likely responsible for the chemotherapeutic effects of N. sativa extract; however, the cellular mechanisms remain ill-defined. TQ-induced cytotoxicity was investigated using canine osteosarcoma (COS31), its cisplatin-resistant variant (COS31/rCDDP), human breast adenocarcinoma (MCF7), human ovarian adenocarcinoma (BG-1) and Madin-Darby canine (MDCK) cell lines. TQ-induced cytotoxicity was determined using a proliferation assay (MTT assay) and apoptosis assays. Effects of TQ on the cell cycle were determined using flow cytometry. COS31/rCDDP resistant cells were the most sensitive cell line to TQ and MDCK cells were the least sensitive. TQ (25 micro M) induced apoptosis of COS31 cells 6 h after treatment and decreased the number of COS31 cells in S-phase and increased cells in G1-phase, indicating cell cycle arrest at G1. These results suggest that TQ kills cancer cells by a process that involves apoptosis and cell cycle arrest. Non-cancerous cells are relatively resistant to TQ.

Original languageEnglish (US)
Pages (from-to)107-113
Number of pages7
JournalInternational journal of oncology
Volume22
Issue number1
StatePublished - Jan 1 2003

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Apoptosis
Cell Line
Growth
Neoplasms
Madin Darby Canine Kidney Cells
Canidae
Adenocarcinoma
G1 Phase Cell Cycle Checkpoints
thymoquinone
In Vitro Techniques
G1 Phase
Osteosarcoma
Cell Cycle Checkpoints
S Phase
Cisplatin
Cell Cycle
Flow Cytometry
Breast
Cell Count

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

In vitro inhibition of growth and induction of apoptosis in cancer cell lines by thymoquinone. / Shoieb, Ahmed M.; Elgayyar, Mona; Dudrick, Paul S.; Bell, John; Tithof, Patricia K.

In: International journal of oncology, Vol. 22, No. 1, 01.01.2003, p. 107-113.

Research output: Contribution to journalArticle

Shoieb, Ahmed M. ; Elgayyar, Mona ; Dudrick, Paul S. ; Bell, John ; Tithof, Patricia K. / In vitro inhibition of growth and induction of apoptosis in cancer cell lines by thymoquinone. In: International journal of oncology. 2003 ; Vol. 22, No. 1. pp. 107-113.
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