In vitro karyotypic and immunophenotypic characterisation of primitive neuroectodermal tumours

Similarities to malignant gliomas

Mark T. Jennings, De Veta L. Jennings, Salah A.D. Ebrahim, Mahlon Johnson, Claude Turc-Carel, Thierry Philip, Irene Philip, Claude Lapras, Joan R. Shapiro

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Monoclonal antibody (Mab) mediated immunotherapy of brain tumours requires the identification of tumour-restricted cell surface antigens. We have characterised four primitive neuroectodermal tumours, which included pineoblastoma, medulloblastoma and ependymoblastoma cultures, that demonstrated in vitro evidence of malignant behaviour (anchorage-independent growth and nu/nu xenograft tumour formation). The cytogenetic findings ranged from normal G-banded and Q-banded karyotypes through mixed near-diploid/hyperdiploid. These cultures resembled the cell surface immunophenotypic spectrum of malignant gliomas. They were distinguished from normal glia in vitro by the expression of restricted fetal mesenchymal, neuronal, myoblastic, melanocytic, epidermal, chondrocytic, lymphoid and epithelial antigens. Certain antigens appeared sufficiently represented among central nervous system (CNS) neoplasms to afford potential targets for Mab-mediated immunotherapy.

Original languageEnglish (US)
Pages (from-to)762-766
Number of pages5
JournalEuropean Journal of Cancer
Volume28
Issue number4-5
DOIs
StatePublished - Jan 1 1992

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Primitive Neuroectodermal Tumors
Glioma
Immunotherapy
Monoclonal Antibodies
Pinealoma
Antigens
Central Nervous System Neoplasms
Medulloblastoma
Polyploidy
Surface Antigens
Diploidy
Karyotype
Heterografts
Brain Neoplasms
Cytogenetics
Neuroglia
Neoplasms
Cell Culture Techniques
Growth
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

In vitro karyotypic and immunophenotypic characterisation of primitive neuroectodermal tumours : Similarities to malignant gliomas. / Jennings, Mark T.; Jennings, De Veta L.; Ebrahim, Salah A.D.; Johnson, Mahlon; Turc-Carel, Claude; Philip, Thierry; Philip, Irene; Lapras, Claude; Shapiro, Joan R.

In: European Journal of Cancer, Vol. 28, No. 4-5, 01.01.1992, p. 762-766.

Research output: Contribution to journalArticle

Jennings, MT, Jennings, DVL, Ebrahim, SAD, Johnson, M, Turc-Carel, C, Philip, T, Philip, I, Lapras, C & Shapiro, JR 1992, 'In vitro karyotypic and immunophenotypic characterisation of primitive neuroectodermal tumours: Similarities to malignant gliomas', European Journal of Cancer, vol. 28, no. 4-5, pp. 762-766. https://doi.org/10.1016/0959-8049(92)90111-E
Jennings, Mark T. ; Jennings, De Veta L. ; Ebrahim, Salah A.D. ; Johnson, Mahlon ; Turc-Carel, Claude ; Philip, Thierry ; Philip, Irene ; Lapras, Claude ; Shapiro, Joan R. / In vitro karyotypic and immunophenotypic characterisation of primitive neuroectodermal tumours : Similarities to malignant gliomas. In: European Journal of Cancer. 1992 ; Vol. 28, No. 4-5. pp. 762-766.
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