In vitro macrophage response to polyethylene and polycarbonate-urethane particles

Richard Smith, Nadim James Hallab

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

This study was undertaken to compare macrophage response to polycarbonate-urethane (PCU), a proposed alternative material to polyethylene in acetabular components of total hip arthroplasty to cross-linked ultrahigh molecular weight polyethylene (xUHMWPE) in the presence or absence of endotoxin. Polyethylene wear debris that is generated by total hip and knee replacements has been linked to osteolysis and limiting the lifespan of the implant. We added both lipopolysaccharide (LPS)-free and endotoxin-associated xUHMWPE and PCU particles to a human monocyte cell line (TH1) in culture and measured cell viability and tumor necrosis factor (TNF)α, interleukin (IL)-1β, and prostaglandin E2 (PGE2) in the medium after 24 h. Results indicate that particles (both xUHMWPE and PCU) free of endotoxin did not significantly induce secretion of TNFα, IL-1β, or PGE2 above basal levels. However, endotoxin-exposed PCU particles induced significantly less TNFa and IL-1β than endotoxin-exposed xUHMWPE particles. This indicates that if endotoxin is available for binding to particles in vivo, then xUHMWPE may be more inflammatory to periprosthetic tissue and bone in part because of its affinity/reactivity with endotoxin when compared with PCU.

Original languageEnglish (US)
Pages (from-to)347-355
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume93
Issue number1
DOIs
StatePublished - Apr 1 2010

Fingerprint

polycarbonate
Macrophages
Urethane
Polyethylene
Polycarbonates
Endotoxins
Polyethylenes
Interleukin-1
Cells
Dinoprostone
Knee prostheses
Arthroplasty
Ultrahigh molecular weight polyethylenes
Tumor Necrosis Factor-alpha
Cell culture
Debris
Bone
Wear of materials
Tissue
Lipopolysaccharides

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

In vitro macrophage response to polyethylene and polycarbonate-urethane particles. / Smith, Richard; Hallab, Nadim James.

In: Journal of Biomedical Materials Research - Part A, Vol. 93, No. 1, 01.04.2010, p. 347-355.

Research output: Contribution to journalArticle

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