In vitro sensitization of human lymphocytes to a myeloma cell-related antigen

M. E. Whitson, G. D. Griffin, G. D. Novelli, Alan Solomon

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Peripheral blood lymphocytes from normal human donors were cocultivated with cells from two established human multiple myeloma cell lines, RPMI 8226 and K-737, and with lymphoblastoid cells from a third B cell line, RAMM. After a comparison of three methods of lymphocyte sensitization, a 6-day incubation protocol with equal numbers of normal lymphocytes and mitomycin C-treated tumor cells was selected. Cells from the RPMI 8226 myeloma line stimulated the differentiation of lymphocytes into cytotoxic effector cells as measured by 51Cr release from labeled target cells. The RPMI 8226-sensitized lymphocytes were cytotoxic for myeloma cells (RPMI 8226 and K-737) and for lymphoblastoid cells (RAMM) but not for cells from human lung tumor lines (A549, A427, MB9812), a breast carcinoma line (ALAB), a normal diploid fibroblast line (HSBP), or normal lymphocytes.

Original languageEnglish (US)
Pages (from-to)489-497
Number of pages9
JournalCellular Immunology
Volume60
Issue number2
DOIs
StatePublished - May 15 1981

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Lymphocytes
Antigens
In Vitro Techniques
Cell Line
Lymphocyte Count
Mitomycin
Diploidy
Multiple Myeloma
Neoplasms
B-Lymphocytes
Fibroblasts
Breast Neoplasms
Lung

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

In vitro sensitization of human lymphocytes to a myeloma cell-related antigen. / Whitson, M. E.; Griffin, G. D.; Novelli, G. D.; Solomon, Alan.

In: Cellular Immunology, Vol. 60, No. 2, 15.05.1981, p. 489-497.

Research output: Contribution to journalArticle

Whitson, M. E. ; Griffin, G. D. ; Novelli, G. D. ; Solomon, Alan. / In vitro sensitization of human lymphocytes to a myeloma cell-related antigen. In: Cellular Immunology. 1981 ; Vol. 60, No. 2. pp. 489-497.
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