In vitro synthesis of Escherichia coli alkaline phosphatase monomers

F Dohan, Robert H. Rubman, Annamaria Torriani

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Messenger RNA-directed in vitro synthesis of Escherichia coli alkaline phosphatase monomers is described. Using a cell-free amino acid incorporation system to which had been added RNA preparations extracted from E. coli constitutively synthesizing alkaline phosphatase, the monomers were synthesized from radioactive amino acids. Immediately after the period of synthesis, the radioactive monomers were captured into alkaline phosphatase dimers by adding a high concentration of non-radioactive monomers. The dimers were then purified away from the other radioactive polypeptides synthesized in the system. An RNA preparation from derepressed wild-type E. coli was active in directing monomer synthesis but RNA extracted from repressed wild-type cells was inactive. The implication of these results regarding the control mechanism of alkaline phosphatase synthesis is discussed.

Original languageEnglish (US)
Pages (from-to)469-479
Number of pages11
JournalJournal of Molecular Biology
Volume58
Issue number2
DOIs
StatePublished - Jun 14 1971
Externally publishedYes

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Alkaline Phosphatase
Escherichia coli
RNA
Amino Acids
Messenger RNA
Peptides
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Cite this

In vitro synthesis of Escherichia coli alkaline phosphatase monomers. / Dohan, F; Rubman, Robert H.; Torriani, Annamaria.

In: Journal of Molecular Biology, Vol. 58, No. 2, 14.06.1971, p. 469-479.

Research output: Contribution to journalArticle

Dohan, F ; Rubman, Robert H. ; Torriani, Annamaria. / In vitro synthesis of Escherichia coli alkaline phosphatase monomers. In: Journal of Molecular Biology. 1971 ; Vol. 58, No. 2. pp. 469-479.
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