In Vivo Simultaneous Tracing and Ca2+ Imaging of Local Neuronal Circuits

Shin Nagayama, Shaoqun Zeng, Wenhui Xiong, Max Fletcher, Arjun V. Masurkar, Douglas J. Davis, Vincent A. Pieribone, Wei R. Chen

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

A central question about the brain is how information is processed by large populations of neurons embedded in intricate local networks. Answering this question requires not only monitoring functional dynamics of many neurons simultaneously, but also interpreting such activity patterns in the context of neuronal circuitry. Here, we introduce a versatile approach for loading Ca2+ indicators in vivo by local electroporation. With this method, Ca2+ imaging can be performed both at neuron population level and with exquisite subcellular resolution down to dendritic spines and axon boutons. This enabled mitral cell odor-evoked ensemble activity to be analyzed simultaneously with revealing their specific connectivity to different glomeruli. Colabeling of Purkinje cell dendrites and intersecting parallel fibers allowed Ca2+ imaging of both presynaptic boutons and postsynaptic dendrites. This approach thus provides an unprecedented capability for in vivo visualizing active cell ensembles and tracing their underlying local neuronal circuits.

Original languageEnglish (US)
Pages (from-to)789-803
Number of pages15
JournalNeuron
Volume53
Issue number6
DOIs
StatePublished - Mar 15 2007
Externally publishedYes

Fingerprint

Dendrites
Neurons
Dendritic Spines
Electroporation
Purkinje Cells
Population
Axons
Brain
Odorants

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Nagayama, S., Zeng, S., Xiong, W., Fletcher, M., Masurkar, A. V., Davis, D. J., ... Chen, W. R. (2007). In Vivo Simultaneous Tracing and Ca2+ Imaging of Local Neuronal Circuits. Neuron, 53(6), 789-803. https://doi.org/10.1016/j.neuron.2007.02.018

In Vivo Simultaneous Tracing and Ca2+ Imaging of Local Neuronal Circuits. / Nagayama, Shin; Zeng, Shaoqun; Xiong, Wenhui; Fletcher, Max; Masurkar, Arjun V.; Davis, Douglas J.; Pieribone, Vincent A.; Chen, Wei R.

In: Neuron, Vol. 53, No. 6, 15.03.2007, p. 789-803.

Research output: Contribution to journalArticle

Nagayama, S, Zeng, S, Xiong, W, Fletcher, M, Masurkar, AV, Davis, DJ, Pieribone, VA & Chen, WR 2007, 'In Vivo Simultaneous Tracing and Ca2+ Imaging of Local Neuronal Circuits', Neuron, vol. 53, no. 6, pp. 789-803. https://doi.org/10.1016/j.neuron.2007.02.018
Nagayama, Shin ; Zeng, Shaoqun ; Xiong, Wenhui ; Fletcher, Max ; Masurkar, Arjun V. ; Davis, Douglas J. ; Pieribone, Vincent A. ; Chen, Wei R. / In Vivo Simultaneous Tracing and Ca2+ Imaging of Local Neuronal Circuits. In: Neuron. 2007 ; Vol. 53, No. 6. pp. 789-803.
@article{af768a45c4f64f94941e4bfd7dd282d5,
title = "In Vivo Simultaneous Tracing and Ca2+ Imaging of Local Neuronal Circuits",
abstract = "A central question about the brain is how information is processed by large populations of neurons embedded in intricate local networks. Answering this question requires not only monitoring functional dynamics of many neurons simultaneously, but also interpreting such activity patterns in the context of neuronal circuitry. Here, we introduce a versatile approach for loading Ca2+ indicators in vivo by local electroporation. With this method, Ca2+ imaging can be performed both at neuron population level and with exquisite subcellular resolution down to dendritic spines and axon boutons. This enabled mitral cell odor-evoked ensemble activity to be analyzed simultaneously with revealing their specific connectivity to different glomeruli. Colabeling of Purkinje cell dendrites and intersecting parallel fibers allowed Ca2+ imaging of both presynaptic boutons and postsynaptic dendrites. This approach thus provides an unprecedented capability for in vivo visualizing active cell ensembles and tracing their underlying local neuronal circuits.",
author = "Shin Nagayama and Shaoqun Zeng and Wenhui Xiong and Max Fletcher and Masurkar, {Arjun V.} and Davis, {Douglas J.} and Pieribone, {Vincent A.} and Chen, {Wei R.}",
year = "2007",
month = "3",
day = "15",
doi = "10.1016/j.neuron.2007.02.018",
language = "English (US)",
volume = "53",
pages = "789--803",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "6",

}

TY - JOUR

T1 - In Vivo Simultaneous Tracing and Ca2+ Imaging of Local Neuronal Circuits

AU - Nagayama, Shin

AU - Zeng, Shaoqun

AU - Xiong, Wenhui

AU - Fletcher, Max

AU - Masurkar, Arjun V.

AU - Davis, Douglas J.

AU - Pieribone, Vincent A.

AU - Chen, Wei R.

PY - 2007/3/15

Y1 - 2007/3/15

N2 - A central question about the brain is how information is processed by large populations of neurons embedded in intricate local networks. Answering this question requires not only monitoring functional dynamics of many neurons simultaneously, but also interpreting such activity patterns in the context of neuronal circuitry. Here, we introduce a versatile approach for loading Ca2+ indicators in vivo by local electroporation. With this method, Ca2+ imaging can be performed both at neuron population level and with exquisite subcellular resolution down to dendritic spines and axon boutons. This enabled mitral cell odor-evoked ensemble activity to be analyzed simultaneously with revealing their specific connectivity to different glomeruli. Colabeling of Purkinje cell dendrites and intersecting parallel fibers allowed Ca2+ imaging of both presynaptic boutons and postsynaptic dendrites. This approach thus provides an unprecedented capability for in vivo visualizing active cell ensembles and tracing their underlying local neuronal circuits.

AB - A central question about the brain is how information is processed by large populations of neurons embedded in intricate local networks. Answering this question requires not only monitoring functional dynamics of many neurons simultaneously, but also interpreting such activity patterns in the context of neuronal circuitry. Here, we introduce a versatile approach for loading Ca2+ indicators in vivo by local electroporation. With this method, Ca2+ imaging can be performed both at neuron population level and with exquisite subcellular resolution down to dendritic spines and axon boutons. This enabled mitral cell odor-evoked ensemble activity to be analyzed simultaneously with revealing their specific connectivity to different glomeruli. Colabeling of Purkinje cell dendrites and intersecting parallel fibers allowed Ca2+ imaging of both presynaptic boutons and postsynaptic dendrites. This approach thus provides an unprecedented capability for in vivo visualizing active cell ensembles and tracing their underlying local neuronal circuits.

UR - http://www.scopus.com/inward/record.url?scp=33847637701&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33847637701&partnerID=8YFLogxK

U2 - 10.1016/j.neuron.2007.02.018

DO - 10.1016/j.neuron.2007.02.018

M3 - Article

VL - 53

SP - 789

EP - 803

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 6

ER -