In vivo SPECT/CT imaging and biodistribution using radioactive Cd 125mTe/ZnS nanoparticles

Jonathan D. Woodward, Stephen Kennel, Saed Mirzadeh, Sheng Dai, Jonathan Wall, Tina Richey, James Avenell, Adam J. Rondinone

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Radioactive cadmium telluride/zinc sulfide (Cd125mTe/ZnS) nanoparticles were targeted to mouse lung with antibody to mouse lung endothelium and quantified using radiological histology in order to test the in vivo targeting efficacy of a nanoparticle-antibody (NP-mAb) system. The nanoparticles were linked to either a monoclonal antibody to mouse lung thrombomodulin (mAb 201B) or a control antibody (mAb 33), and injected into groups of 6-week-old Balb/C female mice. Animals were sacrificed at 1, 4, 24, 72 and 144 h post-injection, and biodistribution in major organs was determined. Full body microSPECT/CT imaging was performed on a pair of mice (experimental and control) providing visual confirmation of the biodistribution. The Cd 125mTe/ZnS NPs conjugated to mAb 201B principally target the lungs while the nanoparticles coupled to mAb 33 accumulate in the liver and spleen. These data provide, for the first time, a quantitative measurement of the in vivo targeting efficacy of an inorganic nanoparticle-mAb system.

Original languageEnglish (US)
Article number175103
JournalNanotechnology
Volume18
Issue number17
DOIs
StatePublished - May 2 2007

Fingerprint

Nanoparticles
Imaging techniques
Antibodies
Cadmium telluride
Zinc sulfide
Thrombomodulin
Histology
Monoclonal antibodies
Liver
Animals
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

In vivo SPECT/CT imaging and biodistribution using radioactive Cd 125mTe/ZnS nanoparticles. / Woodward, Jonathan D.; Kennel, Stephen; Mirzadeh, Saed; Dai, Sheng; Wall, Jonathan; Richey, Tina; Avenell, James; Rondinone, Adam J.

In: Nanotechnology, Vol. 18, No. 17, 175103, 02.05.2007.

Research output: Contribution to journalArticle

Woodward, Jonathan D. ; Kennel, Stephen ; Mirzadeh, Saed ; Dai, Sheng ; Wall, Jonathan ; Richey, Tina ; Avenell, James ; Rondinone, Adam J. / In vivo SPECT/CT imaging and biodistribution using radioactive Cd 125mTe/ZnS nanoparticles. In: Nanotechnology. 2007 ; Vol. 18, No. 17.
@article{15f55eb4dc80428fbec27dd4dde801e2,
title = "In vivo SPECT/CT imaging and biodistribution using radioactive Cd 125mTe/ZnS nanoparticles",
abstract = "Radioactive cadmium telluride/zinc sulfide (Cd125mTe/ZnS) nanoparticles were targeted to mouse lung with antibody to mouse lung endothelium and quantified using radiological histology in order to test the in vivo targeting efficacy of a nanoparticle-antibody (NP-mAb) system. The nanoparticles were linked to either a monoclonal antibody to mouse lung thrombomodulin (mAb 201B) or a control antibody (mAb 33), and injected into groups of 6-week-old Balb/C female mice. Animals were sacrificed at 1, 4, 24, 72 and 144 h post-injection, and biodistribution in major organs was determined. Full body microSPECT/CT imaging was performed on a pair of mice (experimental and control) providing visual confirmation of the biodistribution. The Cd 125mTe/ZnS NPs conjugated to mAb 201B principally target the lungs while the nanoparticles coupled to mAb 33 accumulate in the liver and spleen. These data provide, for the first time, a quantitative measurement of the in vivo targeting efficacy of an inorganic nanoparticle-mAb system.",
author = "Woodward, {Jonathan D.} and Stephen Kennel and Saed Mirzadeh and Sheng Dai and Jonathan Wall and Tina Richey and James Avenell and Rondinone, {Adam J.}",
year = "2007",
month = "5",
day = "2",
doi = "10.1088/0957-4484/18/17/175103",
language = "English (US)",
volume = "18",
journal = "Nanotechnology",
issn = "0957-4484",
publisher = "IOP Publishing Ltd.",
number = "17",

}

TY - JOUR

T1 - In vivo SPECT/CT imaging and biodistribution using radioactive Cd 125mTe/ZnS nanoparticles

AU - Woodward, Jonathan D.

AU - Kennel, Stephen

AU - Mirzadeh, Saed

AU - Dai, Sheng

AU - Wall, Jonathan

AU - Richey, Tina

AU - Avenell, James

AU - Rondinone, Adam J.

PY - 2007/5/2

Y1 - 2007/5/2

N2 - Radioactive cadmium telluride/zinc sulfide (Cd125mTe/ZnS) nanoparticles were targeted to mouse lung with antibody to mouse lung endothelium and quantified using radiological histology in order to test the in vivo targeting efficacy of a nanoparticle-antibody (NP-mAb) system. The nanoparticles were linked to either a monoclonal antibody to mouse lung thrombomodulin (mAb 201B) or a control antibody (mAb 33), and injected into groups of 6-week-old Balb/C female mice. Animals were sacrificed at 1, 4, 24, 72 and 144 h post-injection, and biodistribution in major organs was determined. Full body microSPECT/CT imaging was performed on a pair of mice (experimental and control) providing visual confirmation of the biodistribution. The Cd 125mTe/ZnS NPs conjugated to mAb 201B principally target the lungs while the nanoparticles coupled to mAb 33 accumulate in the liver and spleen. These data provide, for the first time, a quantitative measurement of the in vivo targeting efficacy of an inorganic nanoparticle-mAb system.

AB - Radioactive cadmium telluride/zinc sulfide (Cd125mTe/ZnS) nanoparticles were targeted to mouse lung with antibody to mouse lung endothelium and quantified using radiological histology in order to test the in vivo targeting efficacy of a nanoparticle-antibody (NP-mAb) system. The nanoparticles were linked to either a monoclonal antibody to mouse lung thrombomodulin (mAb 201B) or a control antibody (mAb 33), and injected into groups of 6-week-old Balb/C female mice. Animals were sacrificed at 1, 4, 24, 72 and 144 h post-injection, and biodistribution in major organs was determined. Full body microSPECT/CT imaging was performed on a pair of mice (experimental and control) providing visual confirmation of the biodistribution. The Cd 125mTe/ZnS NPs conjugated to mAb 201B principally target the lungs while the nanoparticles coupled to mAb 33 accumulate in the liver and spleen. These data provide, for the first time, a quantitative measurement of the in vivo targeting efficacy of an inorganic nanoparticle-mAb system.

UR - http://www.scopus.com/inward/record.url?scp=34047274792&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34047274792&partnerID=8YFLogxK

U2 - 10.1088/0957-4484/18/17/175103

DO - 10.1088/0957-4484/18/17/175103

M3 - Article

AN - SCOPUS:34047274792

VL - 18

JO - Nanotechnology

JF - Nanotechnology

SN - 0957-4484

IS - 17

M1 - 175103

ER -