Increased expression and aberrant localization of mucin 13 in metastatic colon cancer

Brij K. Gupta, Diane M. Maher, Mara C. Ebeling, Vasudha Sundram, Michael D. Koch, Douglas W. Lynch, Teresa Bohlmeyer, Akira Watanabe, Hiroyuki Aburatani, Susan E. Puumala, Meena Jaggi, Subhash Chauhan

Research output: Contribution to journalArticle

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Abstract

MUC13 is a newly identified transmembrane mucin. Although MUC13 is known to be overexpressed in ovarian and gastric cancers, limited information is available regarding the expression of MUC13 in metastatic colon cancer. Herein, we investigated the expression profile of MUC13 in colon cancer using a novel anti-MUC13 monoclonal antibody (MAb, clone ppz0020) by immunohistochemical (IHC) analysis. A cohort of colon cancer samples and tissue microarrays containing adjacent normal, non-metastatic colon cancer, metastatic colon cancer, and liver metastasis tissues was used in this study to investigate the expression pattern of MUC13. IHC analysis revealed significantly higher (p<0.001) MUC13 expression in non-metastatic colon cancer samples compared with faint or very low expression in adjacent normal tissues. Interestingly, metastatic colon cancer and liver metastasis tissue samples demonstrated significantly (p<0.05) higher cytoplasmic and nuclear MUC13 expression compared with non-metastatic colon cancer and adjacent normal colon samples. Moreover, cytoplasmic and nuclear MUC13 expression correlated with larger and poorly differentiated tumors. Four of six tested colon cancer cell lines also expressed MUC13 at RNA and protein levels. These studies demonstrate a significant increase in MUC13 expression in metastatic colon cancer and suggest a correlation between aberrant MUC13 localization (cytoplasmic and nuclear expression) and metastatic colon cancer.

Original languageEnglish (US)
Pages (from-to)822-831
Number of pages10
JournalJournal of Histochemistry and Cytochemistry
Volume60
Issue number11
DOIs
StatePublished - Dec 1 2012

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Mucins
Colonic Neoplasms
Liver Neoplasms
Neoplasm Metastasis
Ovarian Neoplasms
Stomach Neoplasms
Colon
Clone Cells
Monoclonal Antibodies
RNA

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology

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Increased expression and aberrant localization of mucin 13 in metastatic colon cancer. / Gupta, Brij K.; Maher, Diane M.; Ebeling, Mara C.; Sundram, Vasudha; Koch, Michael D.; Lynch, Douglas W.; Bohlmeyer, Teresa; Watanabe, Akira; Aburatani, Hiroyuki; Puumala, Susan E.; Jaggi, Meena; Chauhan, Subhash.

In: Journal of Histochemistry and Cytochemistry, Vol. 60, No. 11, 01.12.2012, p. 822-831.

Research output: Contribution to journalArticle

Gupta, BK, Maher, DM, Ebeling, MC, Sundram, V, Koch, MD, Lynch, DW, Bohlmeyer, T, Watanabe, A, Aburatani, H, Puumala, SE, Jaggi, M & Chauhan, S 2012, 'Increased expression and aberrant localization of mucin 13 in metastatic colon cancer', Journal of Histochemistry and Cytochemistry, vol. 60, no. 11, pp. 822-831. https://doi.org/10.1369/0022155412460678
Gupta, Brij K. ; Maher, Diane M. ; Ebeling, Mara C. ; Sundram, Vasudha ; Koch, Michael D. ; Lynch, Douglas W. ; Bohlmeyer, Teresa ; Watanabe, Akira ; Aburatani, Hiroyuki ; Puumala, Susan E. ; Jaggi, Meena ; Chauhan, Subhash. / Increased expression and aberrant localization of mucin 13 in metastatic colon cancer. In: Journal of Histochemistry and Cytochemistry. 2012 ; Vol. 60, No. 11. pp. 822-831.
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