Increased glutathione levels in quiescent, serum‐stimulated NRK‐49F cells are associated not with a response to growth factors but with nutrient repletion

Yujian Kang, M. Duane Enger

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Treatment of quiescent cells with serum results concomitantly in an increase in cellular glutathione (GSH) content and growth stimulation. A possible association between the GSH increase and the growth response was examined by studying separately the effects of nutrients and growth factors on the levels of cellular GSH and proliferation of quiescent NRK‐49F cells. The addition of fresh medium with 10% calf serum was found to result in both a twofold increase in cellular GSH and growth stimulation (DNA synthesis and cell proliferation). 10% calf serum alone, without fresh medium, stimulated cell growth but failed to cause a comparable increase in cellular GSH. The addition of fresh medium without 10% serum, and of 0.5 mM cysteine and glutamate, resulted in both instances in a marked increase in cellular GSH, but failed to stimulate cell growth, EGF, in contrast, induced a complete mitogenic response but did not increase cellular GSH. Finally, pretreatment with L‐buthionine‐(S, R)‐sulfoximine (BSO), a specific inhibitor of GSH synthesis, decreased cellular GSH and inhibited EGF‐induced DNA synthesis, but these two responses do not, in their dose dependency, correlate. The results obtained thus show that the increase in cellular GSH that occurs in quiescent, serum‐stimulated NRK‐49F cells is a result of nutrient repletion rather than mitogenic stimulation, and increased GSH levels do not necessarily precede DNA synthesis and mitosis.

Original languageEnglish (US)
Pages (from-to)197-201
Number of pages5
JournalJournal of Cellular Physiology
Volume148
Issue number2
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

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Nutrients
Glutathione
Intercellular Signaling Peptides and Proteins
Food
Cell growth
Growth
DNA
Serum
Cell Proliferation
Cell proliferation
Epidermal Growth Factor
Cysteine
Glutamic Acid
Mitosis
Cells
Association reactions

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

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title = "Increased glutathione levels in quiescent, serum‐stimulated NRK‐49F cells are associated not with a response to growth factors but with nutrient repletion",
abstract = "Treatment of quiescent cells with serum results concomitantly in an increase in cellular glutathione (GSH) content and growth stimulation. A possible association between the GSH increase and the growth response was examined by studying separately the effects of nutrients and growth factors on the levels of cellular GSH and proliferation of quiescent NRK‐49F cells. The addition of fresh medium with 10{\%} calf serum was found to result in both a twofold increase in cellular GSH and growth stimulation (DNA synthesis and cell proliferation). 10{\%} calf serum alone, without fresh medium, stimulated cell growth but failed to cause a comparable increase in cellular GSH. The addition of fresh medium without 10{\%} serum, and of 0.5 mM cysteine and glutamate, resulted in both instances in a marked increase in cellular GSH, but failed to stimulate cell growth, EGF, in contrast, induced a complete mitogenic response but did not increase cellular GSH. Finally, pretreatment with L‐buthionine‐(S, R)‐sulfoximine (BSO), a specific inhibitor of GSH synthesis, decreased cellular GSH and inhibited EGF‐induced DNA synthesis, but these two responses do not, in their dose dependency, correlate. The results obtained thus show that the increase in cellular GSH that occurs in quiescent, serum‐stimulated NRK‐49F cells is a result of nutrient repletion rather than mitogenic stimulation, and increased GSH levels do not necessarily precede DNA synthesis and mitosis.",
author = "Yujian Kang and {Duane Enger}, M.",
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T1 - Increased glutathione levels in quiescent, serum‐stimulated NRK‐49F cells are associated not with a response to growth factors but with nutrient repletion

AU - Kang, Yujian

AU - Duane Enger, M.

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Y1 - 1991/1/1

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AB - Treatment of quiescent cells with serum results concomitantly in an increase in cellular glutathione (GSH) content and growth stimulation. A possible association between the GSH increase and the growth response was examined by studying separately the effects of nutrients and growth factors on the levels of cellular GSH and proliferation of quiescent NRK‐49F cells. The addition of fresh medium with 10% calf serum was found to result in both a twofold increase in cellular GSH and growth stimulation (DNA synthesis and cell proliferation). 10% calf serum alone, without fresh medium, stimulated cell growth but failed to cause a comparable increase in cellular GSH. The addition of fresh medium without 10% serum, and of 0.5 mM cysteine and glutamate, resulted in both instances in a marked increase in cellular GSH, but failed to stimulate cell growth, EGF, in contrast, induced a complete mitogenic response but did not increase cellular GSH. Finally, pretreatment with L‐buthionine‐(S, R)‐sulfoximine (BSO), a specific inhibitor of GSH synthesis, decreased cellular GSH and inhibited EGF‐induced DNA synthesis, but these two responses do not, in their dose dependency, correlate. The results obtained thus show that the increase in cellular GSH that occurs in quiescent, serum‐stimulated NRK‐49F cells is a result of nutrient repletion rather than mitogenic stimulation, and increased GSH levels do not necessarily precede DNA synthesis and mitosis.

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