Increased hepatic VLDL secretion, lipogenesis, and SREBP-1 expression in the corpulent JCR

LA-cp rat

Marshall Elam, Henry G. Wilcox, Lauren M. Cagen, Xiong Deng, Rajendra Raghow, Poonam Kumar, Murray Heimberg, James C. Russell

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The corpulent JCR:LA-cp rat (cp/cp) is a useful model for study of the metabolic consequences of obesity and hyperinsulinemia. To assess the effect of hyperinsulinemia on VLDL secretion in this model, we measured rates of secretion of VLDL in perfused livers derived from cp/cp rats and their lean littermates. Livers of cp/cp rats secreted significantly greater amounts of VLDL triglyceride and apolipoprotein, compared with lean littermates. The content of apoB, apoE, and apoCs in both perfusate and plasma VLDL was greater in the cp/cp rat, as was the apolipoprotein (apo)C, apoA-I, and apoA-IV content of plasma HDL. Triglyceride content was also greater in cp/cp livers, as was hepatic lipogenesis and expression of lipogenic enzymes and sterol regulatory element binding protein-1 (SREBP-1). Hepatic mRNAs for apoE, and apoA-I were higher in livers of cp/cp rats. In contrast, the steady state levels of apoC-II, apoC-III, and apoB mRNAs were unchanged. Thus, livers of obese hyperinsulinemic cp/cp JCR:LA-cp rats secrete a greater number of VLDL particles that are enriched in triglyceride, apoE, and apoC. Greater secretion of VLDL in the cp/cp rat in part results from higher endogenous fatty acid synthesis, which in turn may occur in response to increased expression of the lipogenic enzyme regulator SREBP-1c.

Original languageEnglish (US)
Pages (from-to)2039-2048
Number of pages10
JournalJournal of Lipid Research
Volume42
Issue number12
StatePublished - 2001

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Sterol Regulatory Element Binding Protein 1
Lipogenesis
Rats
Liver
Apolipoproteins C
Apolipoproteins E
Apolipoprotein A-I
Hyperinsulinism
Apolipoproteins B
Apolipoprotein C-I
Triglycerides
Apolipoprotein C-III
Apolipoproteins A
Plasmas
Messenger RNA
Apolipoproteins
Enzymes
Fatty Acids
Obesity

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Elam, M., Wilcox, H. G., Cagen, L. M., Deng, X., Raghow, R., Kumar, P., ... Russell, J. C. (2001). Increased hepatic VLDL secretion, lipogenesis, and SREBP-1 expression in the corpulent JCR: LA-cp rat. Journal of Lipid Research, 42(12), 2039-2048.

Increased hepatic VLDL secretion, lipogenesis, and SREBP-1 expression in the corpulent JCR : LA-cp rat. / Elam, Marshall; Wilcox, Henry G.; Cagen, Lauren M.; Deng, Xiong; Raghow, Rajendra; Kumar, Poonam; Heimberg, Murray; Russell, James C.

In: Journal of Lipid Research, Vol. 42, No. 12, 2001, p. 2039-2048.

Research output: Contribution to journalArticle

Elam, M, Wilcox, HG, Cagen, LM, Deng, X, Raghow, R, Kumar, P, Heimberg, M & Russell, JC 2001, 'Increased hepatic VLDL secretion, lipogenesis, and SREBP-1 expression in the corpulent JCR: LA-cp rat', Journal of Lipid Research, vol. 42, no. 12, pp. 2039-2048.
Elam, Marshall ; Wilcox, Henry G. ; Cagen, Lauren M. ; Deng, Xiong ; Raghow, Rajendra ; Kumar, Poonam ; Heimberg, Murray ; Russell, James C. / Increased hepatic VLDL secretion, lipogenesis, and SREBP-1 expression in the corpulent JCR : LA-cp rat. In: Journal of Lipid Research. 2001 ; Vol. 42, No. 12. pp. 2039-2048.
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