Increased interleukin-8 expression in inflamed human dental pulps

George Huang, Anthony P. Potente, Jang Wook Kim, Nadia Chugal, Xinli Zhang

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Objective. Elevated levels of interleukin-8, a potent chemoattractant and activator of neutrophils, are associated with infectious and inflammatory diseases. However, little is known about interleukin-8 expression in human dental pulp. The purpose of this study was to determine whether tissue levels of interleukin-8 are elevated in irreversibly inflamed human pulps. Study design. Experimental samples were from teeth clinically diagnosed with irreversible pulpitis (diseased pulps). Controls were from freshly extracted, caries-free third molars (normal pulps). Samples were subjected to enzyme-linked immunosorbent assay and/or immunohistochemical analysis with specific antibodies to interleukin-8. Results. The enzyme-linked immunosorbent assay studies showed elevated levels of interleukin-8 in diseased pulps (mean, 1.82 ± 0.79 pg/mL/μg protein), as compared to detectable interleukin-8 levels in samples from normal pulps (mean, 0.08 ± 0.04 pg/ml/μg protein; P < .05). Immunohistochemical analyses demonstrated that diseased samples exhibited a higher density of localized interleukin-8 staining in areas with heavy infiltration of inflammatory cells. In contrast, normal pulps showed negative or weak interleukin-8 staining. Conclusions. Interleukin-8 concentration was higher in pulps diagnosed with irreversible pulpitis; only negligible amounts of interleukin-8 were present in normal pulps.

Original languageEnglish (US)
Pages (from-to)214-220
Number of pages7
JournalOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
Volume88
Issue number2
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

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Dental Pulp
Interleukin-8
Pulpitis
Enzyme-Linked Immunosorbent Assay
Staining and Labeling
Third Molar
Chemotactic Factors
Communicable Diseases
Tooth
Proteins
Neutrophils

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology
  • Dentistry(all)

Cite this

Increased interleukin-8 expression in inflamed human dental pulps. / Huang, George; Potente, Anthony P.; Kim, Jang Wook; Chugal, Nadia; Zhang, Xinli.

In: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, Vol. 88, No. 2, 01.01.1999, p. 214-220.

Research output: Contribution to journalArticle

Huang, George ; Potente, Anthony P. ; Kim, Jang Wook ; Chugal, Nadia ; Zhang, Xinli. / Increased interleukin-8 expression in inflamed human dental pulps. In: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics. 1999 ; Vol. 88, No. 2. pp. 214-220.
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