Increased presence of oxidized low-density lipoprotein in the left ventricular blood of subjects with cardiovascular disease

Chandrakala Aluganti Narasimhulu, Dmitry Litvinov, Bhaswati Sengupta, Danielle Jones, Chittoor Sai Sudhakar, Michael Firstenberg, Benjamin Sun, Sampath Parthasarathy

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Oxidized LDL (Ox-LDL) and oxidative stress have been implicated in both atherosclerosis and congestive heart failure (HF) development. Here, we tested whether Ox-LDL levels in left ventricular blood (LVB) might differ from those of venous peripheral blood (PB), and whether the level might depend on cardiac function. We also tested whether the LDL molecule is likely to have a longer residence time in the left ventricle of HF subjects with low ejection fraction (EF). The aim of this study was to determine Ox-LDL levels, paraoxonase 1 (PON1) activity, and cholesterol efflux capacity (CEC) of PB and LVB, and correlate these values with LVEF. Sixty-one HF patients underwent preoperative transthoracic echocardiographic assessment of ventricular function. LVEFs were determined using Simpson's biplane technique. LVB and PB levels of Ox-LDL were determined, and PON1 activity and plasma cholesterol efflux capacity were measured. A significant increase in the levels of Ox-LDL in LVB was noted as compared to levels in PB, even when EF was near normal. However, as ejection fraction decreased, the level of Ox-LDL in PB approached that of the LVB. PON1 activity and cholesterol efflux studies indicated increased oxidative stress in LVB and a decreased ability to promote cholesterol efflux from lipid-enriched macrophages. The results suggest that LVB is more oxidatively stressed compared to PB, and therefore LV tissue might be affected differently than peripheral tissues. We recently reported that brain natriuretic peptide (BNP), a marker for HF, is induced by Ox-LDL, so it is possible localized factors within the LV could profoundly affect markers of HF.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalPhysiological Reports
Volume4
Issue number6
DOIs
StatePublished - Mar 1 2016

Fingerprint

Cardiovascular Diseases
Aryldialkylphosphatase
Heart Failure
Cholesterol
oxidized low density lipoprotein
Oxidative Stress
Ventricular Function
Brain Natriuretic Peptide
Heart Ventricles
Atherosclerosis
Macrophages
Lipids

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Increased presence of oxidized low-density lipoprotein in the left ventricular blood of subjects with cardiovascular disease. / Aluganti Narasimhulu, Chandrakala; Litvinov, Dmitry; Sengupta, Bhaswati; Jones, Danielle; Sai Sudhakar, Chittoor; Firstenberg, Michael; Sun, Benjamin; Parthasarathy, Sampath.

In: Physiological Reports, Vol. 4, No. 6, 01.03.2016, p. 1-12.

Research output: Contribution to journalArticle

Aluganti Narasimhulu, C, Litvinov, D, Sengupta, B, Jones, D, Sai Sudhakar, C, Firstenberg, M, Sun, B & Parthasarathy, S 2016, 'Increased presence of oxidized low-density lipoprotein in the left ventricular blood of subjects with cardiovascular disease', Physiological Reports, vol. 4, no. 6, pp. 1-12. https://doi.org/10.14814/phy2.12726
Aluganti Narasimhulu, Chandrakala ; Litvinov, Dmitry ; Sengupta, Bhaswati ; Jones, Danielle ; Sai Sudhakar, Chittoor ; Firstenberg, Michael ; Sun, Benjamin ; Parthasarathy, Sampath. / Increased presence of oxidized low-density lipoprotein in the left ventricular blood of subjects with cardiovascular disease. In: Physiological Reports. 2016 ; Vol. 4, No. 6. pp. 1-12.
@article{367003401cc84d9597c1a938fbca1e9d,
title = "Increased presence of oxidized low-density lipoprotein in the left ventricular blood of subjects with cardiovascular disease",
abstract = "Oxidized LDL (Ox-LDL) and oxidative stress have been implicated in both atherosclerosis and congestive heart failure (HF) development. Here, we tested whether Ox-LDL levels in left ventricular blood (LVB) might differ from those of venous peripheral blood (PB), and whether the level might depend on cardiac function. We also tested whether the LDL molecule is likely to have a longer residence time in the left ventricle of HF subjects with low ejection fraction (EF). The aim of this study was to determine Ox-LDL levels, paraoxonase 1 (PON1) activity, and cholesterol efflux capacity (CEC) of PB and LVB, and correlate these values with LVEF. Sixty-one HF patients underwent preoperative transthoracic echocardiographic assessment of ventricular function. LVEFs were determined using Simpson's biplane technique. LVB and PB levels of Ox-LDL were determined, and PON1 activity and plasma cholesterol efflux capacity were measured. A significant increase in the levels of Ox-LDL in LVB was noted as compared to levels in PB, even when EF was near normal. However, as ejection fraction decreased, the level of Ox-LDL in PB approached that of the LVB. PON1 activity and cholesterol efflux studies indicated increased oxidative stress in LVB and a decreased ability to promote cholesterol efflux from lipid-enriched macrophages. The results suggest that LVB is more oxidatively stressed compared to PB, and therefore LV tissue might be affected differently than peripheral tissues. We recently reported that brain natriuretic peptide (BNP), a marker for HF, is induced by Ox-LDL, so it is possible localized factors within the LV could profoundly affect markers of HF.",
author = "{Aluganti Narasimhulu}, Chandrakala and Dmitry Litvinov and Bhaswati Sengupta and Danielle Jones and {Sai Sudhakar}, Chittoor and Michael Firstenberg and Benjamin Sun and Sampath Parthasarathy",
year = "2016",
month = "3",
day = "1",
doi = "10.14814/phy2.12726",
language = "English (US)",
volume = "4",
pages = "1--12",
journal = "Physiological Reports",
issn = "2051-817X",
publisher = "John Wiley and Sons Inc.",
number = "6",

}

TY - JOUR

T1 - Increased presence of oxidized low-density lipoprotein in the left ventricular blood of subjects with cardiovascular disease

AU - Aluganti Narasimhulu, Chandrakala

AU - Litvinov, Dmitry

AU - Sengupta, Bhaswati

AU - Jones, Danielle

AU - Sai Sudhakar, Chittoor

AU - Firstenberg, Michael

AU - Sun, Benjamin

AU - Parthasarathy, Sampath

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Oxidized LDL (Ox-LDL) and oxidative stress have been implicated in both atherosclerosis and congestive heart failure (HF) development. Here, we tested whether Ox-LDL levels in left ventricular blood (LVB) might differ from those of venous peripheral blood (PB), and whether the level might depend on cardiac function. We also tested whether the LDL molecule is likely to have a longer residence time in the left ventricle of HF subjects with low ejection fraction (EF). The aim of this study was to determine Ox-LDL levels, paraoxonase 1 (PON1) activity, and cholesterol efflux capacity (CEC) of PB and LVB, and correlate these values with LVEF. Sixty-one HF patients underwent preoperative transthoracic echocardiographic assessment of ventricular function. LVEFs were determined using Simpson's biplane technique. LVB and PB levels of Ox-LDL were determined, and PON1 activity and plasma cholesterol efflux capacity were measured. A significant increase in the levels of Ox-LDL in LVB was noted as compared to levels in PB, even when EF was near normal. However, as ejection fraction decreased, the level of Ox-LDL in PB approached that of the LVB. PON1 activity and cholesterol efflux studies indicated increased oxidative stress in LVB and a decreased ability to promote cholesterol efflux from lipid-enriched macrophages. The results suggest that LVB is more oxidatively stressed compared to PB, and therefore LV tissue might be affected differently than peripheral tissues. We recently reported that brain natriuretic peptide (BNP), a marker for HF, is induced by Ox-LDL, so it is possible localized factors within the LV could profoundly affect markers of HF.

AB - Oxidized LDL (Ox-LDL) and oxidative stress have been implicated in both atherosclerosis and congestive heart failure (HF) development. Here, we tested whether Ox-LDL levels in left ventricular blood (LVB) might differ from those of venous peripheral blood (PB), and whether the level might depend on cardiac function. We also tested whether the LDL molecule is likely to have a longer residence time in the left ventricle of HF subjects with low ejection fraction (EF). The aim of this study was to determine Ox-LDL levels, paraoxonase 1 (PON1) activity, and cholesterol efflux capacity (CEC) of PB and LVB, and correlate these values with LVEF. Sixty-one HF patients underwent preoperative transthoracic echocardiographic assessment of ventricular function. LVEFs were determined using Simpson's biplane technique. LVB and PB levels of Ox-LDL were determined, and PON1 activity and plasma cholesterol efflux capacity were measured. A significant increase in the levels of Ox-LDL in LVB was noted as compared to levels in PB, even when EF was near normal. However, as ejection fraction decreased, the level of Ox-LDL in PB approached that of the LVB. PON1 activity and cholesterol efflux studies indicated increased oxidative stress in LVB and a decreased ability to promote cholesterol efflux from lipid-enriched macrophages. The results suggest that LVB is more oxidatively stressed compared to PB, and therefore LV tissue might be affected differently than peripheral tissues. We recently reported that brain natriuretic peptide (BNP), a marker for HF, is induced by Ox-LDL, so it is possible localized factors within the LV could profoundly affect markers of HF.

UR - http://www.scopus.com/inward/record.url?scp=84962091411&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84962091411&partnerID=8YFLogxK

U2 - 10.14814/phy2.12726

DO - 10.14814/phy2.12726

M3 - Article

VL - 4

SP - 1

EP - 12

JO - Physiological Reports

JF - Physiological Reports

SN - 2051-817X

IS - 6

ER -