Increased sensitivity to transient global ischemia in aging rat brain

Kui Xu, Xiaoyan Sun, Michelle Puchowicz, Joseph C. Lamanna

Research output: Chapter in Book/Report/Conference proceedingConference contribution

14 Citations (Scopus)

Abstract

Transient global brain ischemia induced by cardiac arrest and resuscitation (CAR) results in reperfusion injury associated with oxidative stress. Oxidative stress is known to produce delayed selective neuronal cell loss and impairment of brainstem function, leading to post-resuscitation mortality. Levels of 4-hydroxy-2-nonenal (HNE) modified protein adducts, a marker of oxidative stress, was found to be elevated after CAR in rat brain. In this study we investigated the effects of an antioxidant, alpha-phenyl-tert-butyl-nitrone (PBN) on the recovery following CAR in the aged rat brain. Male Fischer 344 rats (6, 12 and 24-month old) underwent 7-minute cardiac arrest before resuscitation. Brainstem function was assessed by hypoxic ventilatory response (HVR) and HNE-adducts were measured by western blot analysis. Our data showed that in the 24-month old rats, overall survival rate, hippocampal CA1 neuronal counts and HVR were significantly reduced compared to the younger rats. With PBN treatment, the recovery was improved in the aged rat brain, which was consistent with reduced HNE adducts in brain following CAR. Our data suggest that aged rats are more vulnerable to oxidative stress insult and treatment with PBN improves the outcome following reperfusion injury. The mechanism of action is most likely through the scavenging of reactive oxygen species resulting in reduced lipid peroxidation.

Original languageEnglish (US)
Title of host publicationOxygen Transport to Tissue XXVIII
PublisherSpringer New York
Pages199-206
Number of pages8
ISBN (Print)9780387717630
DOIs
StatePublished - Jan 1 2008

Publication series

NameAdvances in Experimental Medicine and Biology
Volume599
ISSN (Print)0065-2598

Fingerprint

Resuscitation
Rats
Brain
Ischemia
Aging of materials
Oxidative stress
Heart Arrest
Oxidative Stress
Reperfusion Injury
Brain Stem
Induced Heart Arrest
Inbred F344 Rats
Recovery
Brain Ischemia
Scavenging
Lipid Peroxidation
Reactive Oxygen Species
Survival Rate
Antioxidants
Western Blotting

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Xu, K., Sun, X., Puchowicz, M., & Lamanna, J. C. (2008). Increased sensitivity to transient global ischemia in aging rat brain. In Oxygen Transport to Tissue XXVIII (pp. 199-206). (Advances in Experimental Medicine and Biology; Vol. 599). Springer New York. https://doi.org/10.1007/978-0-387-71764-7_26

Increased sensitivity to transient global ischemia in aging rat brain. / Xu, Kui; Sun, Xiaoyan; Puchowicz, Michelle; Lamanna, Joseph C.

Oxygen Transport to Tissue XXVIII. Springer New York, 2008. p. 199-206 (Advances in Experimental Medicine and Biology; Vol. 599).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Xu, K, Sun, X, Puchowicz, M & Lamanna, JC 2008, Increased sensitivity to transient global ischemia in aging rat brain. in Oxygen Transport to Tissue XXVIII. Advances in Experimental Medicine and Biology, vol. 599, Springer New York, pp. 199-206. https://doi.org/10.1007/978-0-387-71764-7_26
Xu K, Sun X, Puchowicz M, Lamanna JC. Increased sensitivity to transient global ischemia in aging rat brain. In Oxygen Transport to Tissue XXVIII. Springer New York. 2008. p. 199-206. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-0-387-71764-7_26
Xu, Kui ; Sun, Xiaoyan ; Puchowicz, Michelle ; Lamanna, Joseph C. / Increased sensitivity to transient global ischemia in aging rat brain. Oxygen Transport to Tissue XXVIII. Springer New York, 2008. pp. 199-206 (Advances in Experimental Medicine and Biology).
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