Increased spinal monoamine concentrations after chronic thoracic dorsal rhizotomy in goats

Gordon S. Mitchell, Karen B. Bach, Patricia A. Martin, Kevin Foley, E. B. Olson, Mark S. Brownfield, Vjeko Miletic, Mary Behan, Sheila McGuirk, Harry E. Sloan

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

In goats, bilateral thoracic dorsal rhizotomy (TDR) causes severe ventilatory failure during exercise, followed by progressive functional recovery. We investigated spinal neurochemical changes associated with TDR and/or functional recovery by measuring spinal concentrations of the monoamines serotonin (5-HT), norepinephrine, and dopamine via HPLC. Changes in 5-HT and calcitonin gene-related peptide were visualized with immunohistochemistry. Goat spinal cords were compared 4-15 mo after TDR from T 2 to T 12 (n = 7) with sham-operated (n = 4) or unoperated controls (n = 4). TDR increased the concentration of cervical 5-HT (C 5 -C 6 ; 122% change), caudal thoracic norepinephrine (T 7 -T 11 ; 53% change), and rostral thoracic dopamine (T 3 -T 6 ; 234% change). TDR increased 5-HT-immunoreactive terminal density (dorsal and ventral horns) and nearly eliminated calcitonin gene-related peptide immunoreactivity in the superficial laminae of the dorsal horn in rostral thoracic segments; both effects became less pronounced in caudal thoracic segments. Thus TDR elevates monoamine concentrations in discrete spinal regions, including possible compensatory changes in descending serotonergic inputs to spihal segments not directly affected by TDR (i.e., cervical) but associated with functionally related motor nuclei (i.e., phrenic nucleus).

Original languageEnglish (US)
Pages (from-to)1266-1274
Number of pages9
JournalJournal of Applied Physiology
Volume89
Issue number4
StatePublished - Oct 28 2000

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Rhizotomy
Goats
Thorax
Serotonin
Calcitonin Gene-Related Peptide
Dopamine
Norepinephrine
Spinal Cord

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Mitchell, G. S., Bach, K. B., Martin, P. A., Foley, K., Olson, E. B., Brownfield, M. S., ... Sloan, H. E. (2000). Increased spinal monoamine concentrations after chronic thoracic dorsal rhizotomy in goats. Journal of Applied Physiology, 89(4), 1266-1274.

Increased spinal monoamine concentrations after chronic thoracic dorsal rhizotomy in goats. / Mitchell, Gordon S.; Bach, Karen B.; Martin, Patricia A.; Foley, Kevin; Olson, E. B.; Brownfield, Mark S.; Miletic, Vjeko; Behan, Mary; McGuirk, Sheila; Sloan, Harry E.

In: Journal of Applied Physiology, Vol. 89, No. 4, 28.10.2000, p. 1266-1274.

Research output: Contribution to journalArticle

Mitchell, GS, Bach, KB, Martin, PA, Foley, K, Olson, EB, Brownfield, MS, Miletic, V, Behan, M, McGuirk, S & Sloan, HE 2000, 'Increased spinal monoamine concentrations after chronic thoracic dorsal rhizotomy in goats', Journal of Applied Physiology, vol. 89, no. 4, pp. 1266-1274.
Mitchell GS, Bach KB, Martin PA, Foley K, Olson EB, Brownfield MS et al. Increased spinal monoamine concentrations after chronic thoracic dorsal rhizotomy in goats. Journal of Applied Physiology. 2000 Oct 28;89(4):1266-1274.
Mitchell, Gordon S. ; Bach, Karen B. ; Martin, Patricia A. ; Foley, Kevin ; Olson, E. B. ; Brownfield, Mark S. ; Miletic, Vjeko ; Behan, Mary ; McGuirk, Sheila ; Sloan, Harry E. / Increased spinal monoamine concentrations after chronic thoracic dorsal rhizotomy in goats. In: Journal of Applied Physiology. 2000 ; Vol. 89, No. 4. pp. 1266-1274.
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