Increased STAT-3 and synchronous activation of Raf-1-MEK-1-MAPK, and phosphatidylinositol 3-Kinase-Akt-mTOR pathways in atypical and anaplastic meningiomas

Mahlon Johnson, Mary O'Connell, Fran Vito, Robert S. Bakos

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The intracellular events promoting meningioma cell proliferation in high grade tumors are not established. In this study we compared 45 WHO grade I and 35 grade II or III meningiomas by Western blot or immunohistochemistry for phosphorylation/activation of the MEK-1-MAPK, PI3 K-Akt-mTOR-PRAS40 and STAT3 pathways. By Western blot, STAT3 activation was detected in 75% of grade I compared to 100% of grade II and III meningiomas. By immunohistochemistry p-STAT3 was detected in 28% of grade I compared to 65 or 66% of grade II and III meningiomas, respectively. Phosphorylated MEK-1 and p-MAPK were activated in nearly all grade I, II and III tumors. Phosphorylated Akt was also detected in the majority of meningiomas of each grade although downstream pathway component activation was less widespread. These findings suggest that there is increased STAT3 activation in WHO grade II and III meningiomas compared with grade I tumors. Moreover, each of the three major growth regulatory pathways is concomitantly activated in higher grade meningiomas.

Original languageEnglish (US)
Pages (from-to)129-136
Number of pages8
JournalJournal of Neuro-Oncology
Volume92
Issue number2
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

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Phosphatidylinositol 3-Kinase
Mitogen-Activated Protein Kinase Kinases
Meningioma
Western Blotting
Immunohistochemistry
Neoplasms
Phosphorylation
Cell Proliferation
Growth

All Science Journal Classification (ASJC) codes

  • Oncology
  • Neurology
  • Clinical Neurology
  • Cancer Research

Cite this

Increased STAT-3 and synchronous activation of Raf-1-MEK-1-MAPK, and phosphatidylinositol 3-Kinase-Akt-mTOR pathways in atypical and anaplastic meningiomas. / Johnson, Mahlon; O'Connell, Mary; Vito, Fran; Bakos, Robert S.

In: Journal of Neuro-Oncology, Vol. 92, No. 2, 01.01.2009, p. 129-136.

Research output: Contribution to journalArticle

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