Increasing evidence for and regulation of a human placental endogenous digitalis-like factor

Jie Ma, M. Sean Esplin, Charles Adair, Lorrie A. Mason, Steven W. Graves

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Endogenous digitalis-like factors (EDLFs) appear to be hypertensiogenic and increased in the serum and placenta of women with preeclampsia (PE), a complication of pregnancy. Digibind, an anti-digoxin antibody Fab fragment, reverses in vitro effects of EDLF and in vivo features of PE. We used Digibind in a radioimmunoassay to measure EDLF and compared this to a bio-functional assay of EDLF with good agreement. These methods confirmed that human placenta was a source of EDLF, synthesizing and releasing EDLF into the media of cultured human placental tissue. Ketoconazole, a steroid synthesis inhibitor, and 17-OH progesterone, a possible substrate of steroid synthesis, were shown to inhibit or increase EDLF release respectively, suggesting overlap of synthetic pathways. Abnormalities of PE such as placental hypoxia, increased reactive oxygen species and increased pro-inflammatory cytokines were demonstrated to increase placental EDLF release. These findings strongly support placental production of EDLF with increased release due to features of PE.

Original languageEnglish (US)
Pages (from-to)437-448
Number of pages12
JournalReproductive Sciences
Volume19
Issue number4
DOIs
StatePublished - Apr 1 2012

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Pre-Eclampsia
Placenta
digoxin-like factors
Ketoconazole
Pregnancy Complications
Radioimmunoassay
Progesterone
Anti-Idiotypic Antibodies
Reactive Oxygen Species
Steroids
Cytokines
Serum
digoxin antibodies Fab fragments

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynecology

Cite this

Increasing evidence for and regulation of a human placental endogenous digitalis-like factor. / Ma, Jie; Esplin, M. Sean; Adair, Charles; Mason, Lorrie A.; Graves, Steven W.

In: Reproductive Sciences, Vol. 19, No. 4, 01.04.2012, p. 437-448.

Research output: Contribution to journalArticle

Ma, Jie ; Esplin, M. Sean ; Adair, Charles ; Mason, Lorrie A. ; Graves, Steven W. / Increasing evidence for and regulation of a human placental endogenous digitalis-like factor. In: Reproductive Sciences. 2012 ; Vol. 19, No. 4. pp. 437-448.
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