Increasing extracellular potassium results in subthalamic neuron activity resembling that seen in a 6-hydroxydopamine lesion

Ulf Strauss, Fuwen Zhou, Jeannette Henning, Arne Battefeld, Andreas Wree, Rüdiger Köhling, Stefan Jean Pierre Haas, Reiner Benecke, Arndt Rolfs, Ulrike Gimsa

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Abstract

Abnormal neuronal activity in the subthalamic nucleus (STN) plays a crucial role in the pathophysiology of Parkinson's disease (PD). Although altered extracellular potassium concentration ([K+]o) and sensitivity to [K+]o modulates neuronal activity, little is known about the potassium balance in the healthy and diseased STN. In vivo measurements of [K+]o using ion-selective electrodes demonstrated a twofold increase in the decay time constant of lesion-induced [K+]o transients in the STN of adult Wistar rats with a unilateral 6-hydroxydopamine (6-OHDA) median forebrain bundle lesion, employed as a model of PD, compared with nonlesioned rats. Various [K+] o concentrations (1.5-12.5 mM) were applied to in vitro slice preparations of three experimental groups of STN slices from nonlesioned control rats, ipsilateral hemispheres, and contralateral hemispheres of lesioned rats. The majority of STN neurons of nonlesioned rats and in slices contralateral to the lesion fired spontaneously, predominantly in a regular pattern, whereas those in slices ipsilateral to the lesion fired more irregularly or even in bursts. Experimentally increased [K+]o led to an increase in the number of spontaneously firing neurons and action potential firing rates in all groups. This was accompanied by a decrease in the amplitude of post spike afterhyperpolarization (AHP) and the amplitude and duration of the posttrain AHP. Lesion effects in ipsilateral neurons at physiological [K+] o resembled the effects of elevated [K+]o in nonlesioned rats. Our data suggest that changed potassium sensitivity due to conductivity alterations and delayed clearance may be critical for shaping STN activity in parkinsonian states.

Original languageEnglish (US)
Pages (from-to)2902-2915
Number of pages14
JournalJournal of Neurophysiology
Volume99
Issue number6
DOIs
StatePublished - Jun 1 2008

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Subthalamic Nucleus
Oxidopamine
Potassium
Neurons
Parkinson Disease
Medial Forebrain Bundle
Ion-Selective Electrodes
Action Potentials
Wistar Rats

All Science Journal Classification (ASJC) codes

  • Physiology
  • Neuroscience(all)

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Increasing extracellular potassium results in subthalamic neuron activity resembling that seen in a 6-hydroxydopamine lesion. / Strauss, Ulf; Zhou, Fuwen; Henning, Jeannette; Battefeld, Arne; Wree, Andreas; Köhling, Rüdiger; Haas, Stefan Jean Pierre; Benecke, Reiner; Rolfs, Arndt; Gimsa, Ulrike.

In: Journal of Neurophysiology, Vol. 99, No. 6, 01.06.2008, p. 2902-2915.

Research output: Contribution to journalArticle

Strauss, U, Zhou, F, Henning, J, Battefeld, A, Wree, A, Köhling, R, Haas, SJP, Benecke, R, Rolfs, A & Gimsa, U 2008, 'Increasing extracellular potassium results in subthalamic neuron activity resembling that seen in a 6-hydroxydopamine lesion', Journal of Neurophysiology, vol. 99, no. 6, pp. 2902-2915. https://doi.org/10.1152/jn.00402.2007
Strauss, Ulf ; Zhou, Fuwen ; Henning, Jeannette ; Battefeld, Arne ; Wree, Andreas ; Köhling, Rüdiger ; Haas, Stefan Jean Pierre ; Benecke, Reiner ; Rolfs, Arndt ; Gimsa, Ulrike. / Increasing extracellular potassium results in subthalamic neuron activity resembling that seen in a 6-hydroxydopamine lesion. In: Journal of Neurophysiology. 2008 ; Vol. 99, No. 6. pp. 2902-2915.
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