Induction and desensitization of the c-fos mRNA response to nicotine in rat brain

Burt Sharp, H. Stephen Beyer, Kathy M. McAllen, David Hart, Shannon G. Matta

Research output: Contribution to journalArticle

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Abstract

Early rapid response genes such as c-fos are activated in the central nervous system by a variety of agents including psychostimulants. In the present studies, we investigated changes in c-fos mRNA content in several brain regions of the rat in response to nicotine. A single injection of nicotine ip increased the c-fos mRNA content within 30 min and returned toward baseline by 120 min. Significant elevations were induced by 0.5 mg/kg bw nicotine in the medial habenula and by 1.0 mg/kg in the hippocampus, dentate gyrus, and piriform cortex. At 1.0 mg/kg, significantly greater increases in c-fos mRNA levels were present in medial habenula and hippocampus compared to dentate gyrus and in dentate gyrus compared to piriform cortex. Moreover, at 1.0 mg/kg nicotine, increases were significantly less in cerebellar cortex and cingulate gyrus, and these were not dose-dependent. Mecamylamine, a nicotinic cholinergic receptor antagonist, significantly attenuated or eliminated c-fos mRNA response to 1.5 mg/kg nicotine in all regions, except in the cerebellar cortex. Desensitization of the c-fos mRNA response to nicotine was investigated by administering two injections of 2.0 mg/kg nicotine 2 h apart. In the hippocampus, dentate gyrus, and piriform cortex, the first dose of nicotine significantly reduced the c-fos mRNA response to a second dose. The magnitude of desensitization ranged from 43% (piriform cortex) to 81% (hippocampus). In summary, nicotine rapidly elevated the c-fos mRNA content in several rat brain regions. The sensitivity of this response to nicotine and development of desensitization differed among the regions.

Original languageEnglish (US)
Pages (from-to)199-208
Number of pages10
JournalMolecular and Cellular Neurosciences
Volume4
Issue number2
DOIs
StatePublished - Apr 1 1993

Fingerprint

Nicotine
Messenger RNA
Brain
Dentate Gyrus
Hippocampus
Habenula
Cerebellar Cortex
Mecamylamine
Injections
Gyrus Cinguli
Nicotinic Receptors
Cholinergic Antagonists
Central Nervous System
Piriform Cortex

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Induction and desensitization of the c-fos mRNA response to nicotine in rat brain. / Sharp, Burt; Beyer, H. Stephen; McAllen, Kathy M.; Hart, David; Matta, Shannon G.

In: Molecular and Cellular Neurosciences, Vol. 4, No. 2, 01.04.1993, p. 199-208.

Research output: Contribution to journalArticle

Sharp, Burt ; Beyer, H. Stephen ; McAllen, Kathy M. ; Hart, David ; Matta, Shannon G. / Induction and desensitization of the c-fos mRNA response to nicotine in rat brain. In: Molecular and Cellular Neurosciences. 1993 ; Vol. 4, No. 2. pp. 199-208.
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