Induction of antioxidants by adriamycin in mouse heart

Xianhua Yin, Huiyun Wu, Yan Chen, Yujian Kang

Research output: Contribution to journalArticle

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Abstract

Cardiac oxidative injury is a major limiting factor for clinical application of Adriamycin (ADR) in cancer chemotherapy. ADR depresses some antioxidant systems, thereby further enhancing the cardiotoxicity. Previous studies have shown that ADR inhibits the overall synthesis of DNA, RNA, and protein. It was presumed that the depressed antioxidant activity resulted from the inhibited gene expression. However, there were no experimental data to demonstrate the relationship between the change in antioxidant activities and that in their gene expression. Therefore, the present study was undertaken to examine the effects of ADR on the activities and mRNA abundances of antioxidants in mouse heart. FVB mice (7 weeks old) were treated with ADR (15 mg/kg) by a single i.p. injection. Four days after the treatment, cardiac antioxidant activities and mRNA abundances were measured. The results showed that ADR increased the levels of mRNAs for Cu,Zn-superoxide dismutase (Cu,Zn-SOD), catalase, glutathione peroxidase (GSHpx), and γ-glutamylcysteine synthetase (γ-GCS). On the other hand, ADR increased the activities of catalase and γ-GCS, and slightly decreased total glutathione concentrations in the heart. Cu,Zn-superoxide dismutase, Mn-superoxide dismutase, and glutathione peroxidase activities were not changed significantly. In addition, ADR increased both mRNA and protein levels of metallothionein in the heart. The data demonstrate that up-regulation of antioxidant gene expression occurred in response to ADR in the mouse heart, although the antioxidant activities were not all increased. Copyright (C) 1998 Elsevier Science, Inc.

Original languageEnglish (US)
Pages (from-to)87-93
Number of pages7
JournalBiochemical Pharmacology
Volume56
Issue number1
DOIs
StatePublished - Jul 1 1998
Externally publishedYes

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Doxorubicin
Antioxidants
Gene expression
Messenger RNA
Glutathione Peroxidase
Catalase
Glutamate-Cysteine Ligase
Gene Expression
Chemotherapy
Metallothionein
Gene Expression Regulation
Superoxide Dismutase
Glutathione
Proteins
Up-Regulation
RNA
Drug Therapy
Injections
DNA
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Pharmacology

Cite this

Induction of antioxidants by adriamycin in mouse heart. / Yin, Xianhua; Wu, Huiyun; Chen, Yan; Kang, Yujian.

In: Biochemical Pharmacology, Vol. 56, No. 1, 01.07.1998, p. 87-93.

Research output: Contribution to journalArticle

Yin, Xianhua ; Wu, Huiyun ; Chen, Yan ; Kang, Yujian. / Induction of antioxidants by adriamycin in mouse heart. In: Biochemical Pharmacology. 1998 ; Vol. 56, No. 1. pp. 87-93.
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