Induction of brain natriuretic peptide and monocyte chemotactic Protein-1 gene expression by oxidized low-density lipoprotein

Relevance to ischemic heart failure

Aluganti N. Chandrakala, Devraj Sukul, Krithika Selvarajan, Chittoor Sai Sudhakar, Benjamin Sun, Sampath Parthasarathy

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Brain natriuretic peptide (BNP) and monocyte chemotactic protein-1 (MCP-1) are biomarkers of heart failure (HF). The aim of the present study was to determine the role of oxidized low-density lipoprotein (Ox-LDL) in the induction of these biomarkers and the signaling pathways involved in vitro. Incubation of HL-1 cardiomyocytes and human myocytes with Ox-LDL induced the expression of BNP and MCP-1 genes, while native LDL had no effect. When peroxides associated with Ox-LDL were reduced to hydroxides, the ability to induce BNP and MCP-1 gene expression was abolished. Furthermore, exposure of HL-1 cells to ischemic conditions alone had no effect on BNP gene expression, while ischemia followed by reperfusion resulted in increased expression of BNP gene. Inhibitors of ERK and JNK inhibited the induction of BNP. Signaling array results suggested that the induction of both MAPK and NF-κB pathways is involved in the induction of BNP by Ox-LDL. These results suggest that Ox-LDL or peroxidized lipids formed in oxidatively stressed myocytes during ischemia-reperfusion injury may play a role in the induction of BNP and MCP-1.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume302
Issue number1
DOIs
StatePublished - Jan 1 2012
Externally publishedYes

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Chemokine CCL2
Brain Natriuretic Peptide
Heart Failure
Gene Expression
Muscle Cells
Biomarkers
Hydroxides
oxidized low density lipoprotein
Peroxides
Reperfusion Injury
Cardiac Myocytes
Genes
Reperfusion
Ischemia
Lipids

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cell Biology

Cite this

Induction of brain natriuretic peptide and monocyte chemotactic Protein-1 gene expression by oxidized low-density lipoprotein : Relevance to ischemic heart failure. / Chandrakala, Aluganti N.; Sukul, Devraj; Selvarajan, Krithika; Sai Sudhakar, Chittoor; Sun, Benjamin; Parthasarathy, Sampath.

In: American Journal of Physiology - Cell Physiology, Vol. 302, No. 1, 01.01.2012.

Research output: Contribution to journalArticle

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