Induction of fibroblast proliferation by interleukin‐1 derived from human monocytic leukemia cells

Arnold Postlethwaite, Lawrence B. Lachman, Andrew Kang

Research output: Contribution to journalArticle

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Abstract

Human interleukin‐1 (IL‐1), free of contaminating lymphokines, was isolated from cultures of purified monoblasts from a patient with acute monocytic leukemia. Partially purified IL‐1 (diafiltration, ultrafiltration, and isoelectric focusing) stimulated proliferation of subconfluent human fibroblasts in vitro. Further purification of IL‐1 by high‐resolution gel filtration‐ and anion exchange‐high performance liquid chromatography revealed that fibroblast proliferation activity could not be separated from IL‐1 activity (thymocyte proliferation), suggesting that both activities are the properties of a single molecule. Fibroblasts and thymocytes exhibited a similar sensitivity to the proliferative effects of IL‐1. These findings suggest that macrophages participating in inflammatory reactions in vivo might release IL‐1, which could function to expand fibroblast populations at sites of inflammatory reactions, by acting as a fibroblast growth factor.

Original languageEnglish (US)
Pages (from-to)995-1001
Number of pages7
JournalArthritis & Rheumatism
Volume27
Issue number9
DOIs
StatePublished - Jan 1 1984

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Leukemia
Fibroblasts
Thymocytes
Monocyte-Macrophage Precursor Cells
Leukemia, Monocytic, Acute
Fibroblast Growth Factors
Lymphokines
Ultrafiltration
Isoelectric Focusing
Liquid Chromatography
Gel Chromatography
Anions
Macrophages
Population

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

Cite this

Induction of fibroblast proliferation by interleukin‐1 derived from human monocytic leukemia cells. / Postlethwaite, Arnold; Lachman, Lawrence B.; Kang, Andrew.

In: Arthritis & Rheumatism, Vol. 27, No. 9, 01.01.1984, p. 995-1001.

Research output: Contribution to journalArticle

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