Induction of tolerance by oral administration of beta-tubulin in an animal model of autoimmune inner ear disease

Qing Cai, Xiaoping Du, Bin Zhou, Chun Cai, Mohammad Habiby Kermany, Taijune Yoo

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Induction of peripheral tolerance by oral administration of low-dose β-tubulin antigen may be an effective, antigen-specific method to suppress experimental autoimmune hearing loss. Five groups of mice were fed with phosphate-buffered saline (PBS), ovalbumin (OVA), 20, 30 or 200 μg of β-tubulin, respectively. All mice were then immunized by β-tubulin. Hearing thresholds were measured before and after immunization. Inner ear histology and cytokine profile were examined. Mice fed with 20 or 30 μg of β-tubulin showed less hearing loss and less inner ear damage compared to the groups treated with PBS, OVA or 200 μg of β-tubulin. Interferon-gamma (IFN-γ) was decreased while interleukin-4 (IL-4), IL-5, IL-13 and TGF-β were increased in both sera and in cell culture supernatants of the mice fed with 20 or 30 μg of β-tubulin. However, no cytokine profile change was found in the group treated with 200 μg of tubulin. These results suggest that a low dose of β-tubulin is active orally in an antigen-specific fashion and capable of inhibiting the autoimmune reactions in the inner ear by suppressingTh1 (IFN-γ) and increasing Th2 and Th3 (IL-4, IL-5, IL-13 and TGF-β) cytokines. Oral antigen tolerance may be used to treat autoimmune inner ear disease.

Original languageEnglish (US)
Pages (from-to)135-141
Number of pages7
JournalORL
Volume71
Issue number3
DOIs
StatePublished - Jul 1 2009

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Labyrinth Diseases
Tubulin
Oral Administration
Animal Models
Inner Ear
Antigens
Interleukin-13
Interleukin-5
Ovalbumin
Cytokines
Hearing Loss
Interleukin-4
Interferon-gamma
Phosphates
Peripheral Tolerance
Hearing
Immunization
Histology
Cell Culture Techniques

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

Cite this

Induction of tolerance by oral administration of beta-tubulin in an animal model of autoimmune inner ear disease. / Cai, Qing; Du, Xiaoping; Zhou, Bin; Cai, Chun; Kermany, Mohammad Habiby; Yoo, Taijune.

In: ORL, Vol. 71, No. 3, 01.07.2009, p. 135-141.

Research output: Contribution to journalArticle

Cai, Qing ; Du, Xiaoping ; Zhou, Bin ; Cai, Chun ; Kermany, Mohammad Habiby ; Yoo, Taijune. / Induction of tolerance by oral administration of beta-tubulin in an animal model of autoimmune inner ear disease. In: ORL. 2009 ; Vol. 71, No. 3. pp. 135-141.
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