Inflammatory cytokines as potential biomarkers for damage in total knee arthroplasty

Meredith Perkins, Julie Lowell, Anita Kerkhof, William Mihalko

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Inflammatory cytokines have been proposed as potential biomarkers for damage in total knee arthroplasty (TKA). This study sought to compare the levels of inflammatory cytokines (TNF-α, IL-lβ, IL-6, IL-8, MCP-1, MIP-1α, MIP-3α, GM-CSF, and M-CSF) in synovial fluid of retrieved cadaveric primary TKAs, painful TKAs, and failed TKAs obtained at the time of revision. Twenty-five cadaveric specimens with primary TKAs were procured, and synovial fluid was collected. Seven synovial aspirates were collected during revision surgery from patients with failed primary TKAs, and twelve synovial aspirates were collected during clinic visits from patients with painful primary TKAs. Synovial samples were analyzed using a premixed Luminex Multiplex Screening Assay kit for detection of human inflammatory cytokines. A Kruskal-Wallis statistical test with Dunn's multiple comparison post hoc test and an assumed significance (p < 0.05) was used. Statistical analysis revealed a significant difference (p = 0.028) between IL-6 concentrations present in painful and cadaveric samples. No significant difference (p = 0.343) was found among the mean MCP-1 concentrations across the three sample groups. The cadaveric and painful samples had elevated MIP-3α compared to the revision samples upon initial inspection. Statistical analysis revealed a significant difference between cadaveric and revision sample groups. The cadaveric specimens had concentrations that were significantly elevated in comparison to the painful (p < 0.0001) and revision samples (p = 0.0015). IL-6 may be a potential biomarker for damage in a TKA. To better understand the role of MIP-3α, a future study should increase the sample size of the painful and revision groups. Future research will investigate the role of M-CSF concentrations as indicators in progression of TKA failure. Understanding the roles of these inflammatory cytokines throughout the progression of primary TKA complications may improve the diagnosis and treatment of painful TKAs.

Original languageEnglish (US)
Title of host publicationBeyond the Implant
Subtitle of host publicationRetrieval Analysis Methods for Implant Surveillance
EditorsA. Seth Greenwald, William M. Mihalko, Steven M. Kurtz, Jack E. Lemons
PublisherASTM International
Pages191-202
Number of pages12
VolumeSTP 1606
ISBN (Print)9780803176577
DOIs
StatePublished - Jan 1 2018
EventSymposium on Beyond the Implant: Retrieval Analysis Methods for Implant Surveillance - Toronto, Canada
Duration: May 9 2017 → …

Other

OtherSymposium on Beyond the Implant: Retrieval Analysis Methods for Implant Surveillance
CountryCanada
CityToronto
Period5/9/17 → …

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Arthroplasty
Biomarkers
Macrophage Colony-Stimulating Factor
Cytokines
Interleukin-6
Statistical methods
Fluids
Statistical tests
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-8
Surgery
Assays
Screening
Inspection

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Perkins, M., Lowell, J., Kerkhof, A., & Mihalko, W. (2018). Inflammatory cytokines as potential biomarkers for damage in total knee arthroplasty. In A. S. Greenwald, W. M. Mihalko, S. M. Kurtz, & J. E. Lemons (Eds.), Beyond the Implant: Retrieval Analysis Methods for Implant Surveillance (Vol. STP 1606, pp. 191-202). ASTM International. https://doi.org/10.1520/STP160620170100

Inflammatory cytokines as potential biomarkers for damage in total knee arthroplasty. / Perkins, Meredith; Lowell, Julie; Kerkhof, Anita; Mihalko, William.

Beyond the Implant: Retrieval Analysis Methods for Implant Surveillance. ed. / A. Seth Greenwald; William M. Mihalko; Steven M. Kurtz; Jack E. Lemons. Vol. STP 1606 ASTM International, 2018. p. 191-202.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Perkins, M, Lowell, J, Kerkhof, A & Mihalko, W 2018, Inflammatory cytokines as potential biomarkers for damage in total knee arthroplasty. in AS Greenwald, WM Mihalko, SM Kurtz & JE Lemons (eds), Beyond the Implant: Retrieval Analysis Methods for Implant Surveillance. vol. STP 1606, ASTM International, pp. 191-202, Symposium on Beyond the Implant: Retrieval Analysis Methods for Implant Surveillance, Toronto, Canada, 5/9/17. https://doi.org/10.1520/STP160620170100
Perkins M, Lowell J, Kerkhof A, Mihalko W. Inflammatory cytokines as potential biomarkers for damage in total knee arthroplasty. In Greenwald AS, Mihalko WM, Kurtz SM, Lemons JE, editors, Beyond the Implant: Retrieval Analysis Methods for Implant Surveillance. Vol. STP 1606. ASTM International. 2018. p. 191-202 https://doi.org/10.1520/STP160620170100
Perkins, Meredith ; Lowell, Julie ; Kerkhof, Anita ; Mihalko, William. / Inflammatory cytokines as potential biomarkers for damage in total knee arthroplasty. Beyond the Implant: Retrieval Analysis Methods for Implant Surveillance. editor / A. Seth Greenwald ; William M. Mihalko ; Steven M. Kurtz ; Jack E. Lemons. Vol. STP 1606 ASTM International, 2018. pp. 191-202
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