Influence of age, sex, and strength training on human muscle gene expression determined by microarray

Stephen M. Roth, Robert E. Ferrell, David G. Peters, E. Metter, Ben F. Hurley, Marc A. Rogers

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

The purpose of this study was to determine the influence of age, sex, and strength training (ST) on large-scale gene expression patterns in vastus lateralis muscle biopsies using high-density cDNA microarrays and quantitative PCR. Muscle samples from sedentary young (20-30 yr) and older (65-75 yr) men and women (5 per group) were obtained before and after a 9-wk unilateral heavy resistance ST program. RNA was hybridized to cDNA filter microarrays representing ∼4,000 known human genes and comparisons were made among arrays to determine differential gene expression as a result of age and sex differences, and/or response to ST. Sex had the strongest influence on muscle gene expression, with differential expression (>1.7-fold) observed for ∼200 genes between men and women (∼75% with higher expression in men). Age contributed to differential expression as well, as ∼50 genes were identified as differentially expressed (>1.7-fold) in relation to age, representing structural, metabolic, and regulatory gene classes. Sixty-nine genes were identified as being differentially expressed (>1.7-fold) in all groups in response to ST, and the majority of these were downregulated. Quantitative PCR was employed to validate expression levels for caldesmon, SWI/SNF (BAF60b), and four-and-a-half LIM domains 1. These significant differences suggest that in the analysis of skeletal muscle gene expression issues of sex, age, and habitual physical activity must be addressed, with sex being the most critical variable.

Original languageEnglish (US)
Pages (from-to)181-190
Number of pages10
JournalPhysiological genomics
Volume2002
Issue number10
StatePublished - Jan 1 2002
Externally publishedYes

Fingerprint

Resistance Training
Gene Expression
Muscles
Oligonucleotide Array Sequence Analysis
Genes
Calmodulin-Binding Proteins
Polymerase Chain Reaction
Quadriceps Muscle
Regulator Genes
Sex Characteristics
Skeletal Muscle
Down-Regulation
RNA
Exercise
Biopsy
Education

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics

Cite this

Roth, S. M., Ferrell, R. E., Peters, D. G., Metter, E., Hurley, B. F., & Rogers, M. A. (2002). Influence of age, sex, and strength training on human muscle gene expression determined by microarray. Physiological genomics, 2002(10), 181-190.

Influence of age, sex, and strength training on human muscle gene expression determined by microarray. / Roth, Stephen M.; Ferrell, Robert E.; Peters, David G.; Metter, E.; Hurley, Ben F.; Rogers, Marc A.

In: Physiological genomics, Vol. 2002, No. 10, 01.01.2002, p. 181-190.

Research output: Contribution to journalArticle

Roth, SM, Ferrell, RE, Peters, DG, Metter, E, Hurley, BF & Rogers, MA 2002, 'Influence of age, sex, and strength training on human muscle gene expression determined by microarray', Physiological genomics, vol. 2002, no. 10, pp. 181-190.
Roth, Stephen M. ; Ferrell, Robert E. ; Peters, David G. ; Metter, E. ; Hurley, Ben F. ; Rogers, Marc A. / Influence of age, sex, and strength training on human muscle gene expression determined by microarray. In: Physiological genomics. 2002 ; Vol. 2002, No. 10. pp. 181-190.
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