Influence of phospholipid composition on the properties of reconstituted surfactants

R. Dhand, J. Young, S. Krishnasamy, F. Possmayer, N. J. Gross

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The influence of phospholipids on the ultrastructure and metabolism of reconstituted surfactants has not been well defined. The aim of this study was to determine if changes in the phospholipid composition of reconstituted surfactants altered their biophysical properties, ultrastructure, and conversion to light subtype by cycling. We prepared various surfactants containing radiolabeled dipalmitoylphosphatidylcholine ([14C]DPPC). The addition of phosphatidylglycerol (PG) or dipalmitoylphosphatidic acid (PA) to DPPC increased conversion to light subtype. In contrast, the addition of dipalmitoylphosphatidylglycerol (DPPG) to DPPC markedly reduced conversion to light subtype on cycling. DPPC and DPPC+PG produced large liposomes (~1,000 nm), whereas DPPC+PA or DPPC+DPPG formed multilamellar membranes. Mixtures of DPPC and PA were highly surface active in vitro, whereas the surface activity of DPPC+DPPG was similar to that of DPPC. In conclusion, the ultrastructure, metabolism, and surface active properties of DPPC+PG mixtures were influenced markedly by alterations in the fatty acid composition or polar head group of PG.

Original languageEnglish (US)
Pages (from-to)127-138
Number of pages12
JournalLung
Volume177
Issue number3
DOIs
StatePublished - May 11 1999

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Phosphatidylglycerols
Surface-Active Agents
Phospholipids
Light
1,2-Dipalmitoylphosphatidylcholine
Surface Properties
Liposomes
Fatty Acids
Membranes
1,2-dipalmitoylphosphatidylglycerol

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

Cite this

Dhand, R., Young, J., Krishnasamy, S., Possmayer, F., & Gross, N. J. (1999). Influence of phospholipid composition on the properties of reconstituted surfactants. Lung, 177(3), 127-138. https://doi.org/10.1007/PL00007634

Influence of phospholipid composition on the properties of reconstituted surfactants. / Dhand, R.; Young, J.; Krishnasamy, S.; Possmayer, F.; Gross, N. J.

In: Lung, Vol. 177, No. 3, 11.05.1999, p. 127-138.

Research output: Contribution to journalArticle

Dhand, R, Young, J, Krishnasamy, S, Possmayer, F & Gross, NJ 1999, 'Influence of phospholipid composition on the properties of reconstituted surfactants', Lung, vol. 177, no. 3, pp. 127-138. https://doi.org/10.1007/PL00007634
Dhand, R. ; Young, J. ; Krishnasamy, S. ; Possmayer, F. ; Gross, N. J. / Influence of phospholipid composition on the properties of reconstituted surfactants. In: Lung. 1999 ; Vol. 177, No. 3. pp. 127-138.
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