Inhibition of cell-free protein synthesis initiation by amphetamine

Association with reduction in tRNA aminoacylation

Thaddeus Nowak, Hamish N. Munro

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Using homologous initiating and non-initiating protein synthesis systems prepared from rabbit reticulocytes, it was found that 4 to 10 mM amphetamine preferentially inhibited initiation-dependent amino acid incorporation without affecting elongation, in agreement with previous results using wheat germ initiating and rat elongating systems. A test of several para-substituted amphetamines yielded an order of potency pOH < pF ≅ amphetamine < pCl < pBr. Over the same concentration range at which these compounds inhibited protein synthesis, they proportionately inhibited aminoacylation of tRNA, suggesting that this may be responsible for their inhibitory action on protein synthesis.

Original languageEnglish (US)
Pages (from-to)1280-1285
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume77
Issue number4
DOIs
StatePublished - Aug 22 1977

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Transfer RNA Aminoacylation
Amphetamine
Transfer RNA
Cells
Amphetamines
Proteins
Reticulocytes
Triticum
Rats
Elongation
Rabbits
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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AB - Using homologous initiating and non-initiating protein synthesis systems prepared from rabbit reticulocytes, it was found that 4 to 10 mM amphetamine preferentially inhibited initiation-dependent amino acid incorporation without affecting elongation, in agreement with previous results using wheat germ initiating and rat elongating systems. A test of several para-substituted amphetamines yielded an order of potency pOH < pF ≅ amphetamine < pCl < pBr. Over the same concentration range at which these compounds inhibited protein synthesis, they proportionately inhibited aminoacylation of tRNA, suggesting that this may be responsible for their inhibitory action on protein synthesis.

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