Inhibition of DNA synthesis in Caco-2 cells by oxidative stress amelioration by epidermal growth factor

J. A. Engler, A. Gupta, Radhakrishna Rao

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The role of oxidative stress in the regulation of intestinal epithelial proliferation was examined by evaluating the effect of H2O2 and xanthine oxidase + xanthine (XO + X) on [3H]thymidine incorporation into DNA in Caco- 2 cells. DNA synthesis was highest 4 and 5 days after seeding, while it declined rapidly between 5 and 12 days. Pretreatment for 0.5-24 h with H2O2 or XO + X reduced DNA synthesis on 4- to 6-day-old, but not on 7- to 20-day- old cells. The effect of XO + X on DNA synthesis was significantly reduced by catalase, superoxide dismutase, and ferric chloride, but pretreatment with deferoxamine potentiated XO + X-induced inhibition of DNA synthesis. Coadministration of epidermal growth factor (EGF) for 24 hr reduced the H2O2 and XO + X-induced inhibition of DNA synthesis; this effect of EGF was not observed up to 8 hr. Results show that O2 and H2O2 rapidly inhibit DNA synthesis in Caco-2 cells and that EGF restores DNA synthesis in oxidant- treated cells.

Original languageEnglish (US)
Pages (from-to)1902-1909
Number of pages8
JournalDigestive Diseases and Sciences
Volume44
Issue number9
DOIs
StatePublished - Oct 1 1999

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Caco-2 Cells
Epidermal Growth Factor
Oxidative Stress
Xanthine
Xanthine Oxidase
DNA
Deferoxamine
Oxidants
Catalase
Thymidine
Superoxide Dismutase

All Science Journal Classification (ASJC) codes

  • Physiology
  • Gastroenterology

Cite this

Inhibition of DNA synthesis in Caco-2 cells by oxidative stress amelioration by epidermal growth factor. / Engler, J. A.; Gupta, A.; Rao, Radhakrishna.

In: Digestive Diseases and Sciences, Vol. 44, No. 9, 01.10.1999, p. 1902-1909.

Research output: Contribution to journalArticle

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