Ingibirovanie ékzogennykh serinovykh proteinaz ingibitorom tripsina iz semian grechikhi IT-1.

Translated title of the contribution: Inhibition of exogenous serine proteinases by a trypsin inhibitor from the buckwheat IT-1 seeds

I. P. Gladysheva, I. E. Dunaevskii, M. A. Belozerskii, D. P. Gladyshev, A. I. Papisova, N. I. Larionova

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The possibility of inhibition of exogenous trypsin- and chymotrypsin-like proteinases by a proteinase inhibitor from buckwheat (IT-1) seeds has been studied. The inhibition constants for bovine trypsin and alpha-chymotrypsin and human granulocyte cathepsin G by IT-1 are equal to 1.1, 67 and 200 nm, respectively. The specificity of IT-1 with regard to its primary sequence adjacent to the active center and to its homology with inhibitors pertaining to the potato inhibitor I family has been carried out. It is concluded that by virtue of the basic nature of the P1 (Arg) residue in the active center IT-1 is not capable to bind human granulocyte elastase.

Original languageRussian
Pages (from-to)1530-1535
Number of pages6
JournalBiokhimiia (Moscow, Russia)
Volume60
Issue number9
StatePublished - Sep 1 1995

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Cathepsin G
Leukocyte Elastase
Trypsin Inhibitors
Serine Proteases
Trypsin
Seed
Peptide Hydrolases
trypsin drug combination chymotrypsin
alpha-chymotrypsin
aspergillopepsin II

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Gladysheva, I. P., Dunaevskii, I. E., Belozerskii, M. A., Gladyshev, D. P., Papisova, A. I., & Larionova, N. I. (1995). Ingibirovanie ékzogennykh serinovykh proteinaz ingibitorom tripsina iz semian grechikhi IT-1. Biokhimiia (Moscow, Russia), 60(9), 1530-1535.

Ingibirovanie ékzogennykh serinovykh proteinaz ingibitorom tripsina iz semian grechikhi IT-1. / Gladysheva, I. P.; Dunaevskii, I. E.; Belozerskii, M. A.; Gladyshev, D. P.; Papisova, A. I.; Larionova, N. I.

In: Biokhimiia (Moscow, Russia), Vol. 60, No. 9, 01.09.1995, p. 1530-1535.

Research output: Contribution to journalArticle

Gladysheva, IP, Dunaevskii, IE, Belozerskii, MA, Gladyshev, DP, Papisova, AI & Larionova, NI 1995, 'Ingibirovanie ékzogennykh serinovykh proteinaz ingibitorom tripsina iz semian grechikhi IT-1.', Biokhimiia (Moscow, Russia), vol. 60, no. 9, pp. 1530-1535.
Gladysheva IP, Dunaevskii IE, Belozerskii MA, Gladyshev DP, Papisova AI, Larionova NI. Ingibirovanie ékzogennykh serinovykh proteinaz ingibitorom tripsina iz semian grechikhi IT-1. Biokhimiia (Moscow, Russia). 1995 Sep 1;60(9):1530-1535.
Gladysheva, I. P. ; Dunaevskii, I. E. ; Belozerskii, M. A. ; Gladyshev, D. P. ; Papisova, A. I. ; Larionova, N. I. / Ingibirovanie ékzogennykh serinovykh proteinaz ingibitorom tripsina iz semian grechikhi IT-1. In: Biokhimiia (Moscow, Russia). 1995 ; Vol. 60, No. 9. pp. 1530-1535.
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