Inhibition of hypoxia-induced cell motility by p16 in MDA-MB-231 breast cancer cells

Liyuan Li, Yi Lu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Our previous studies indicated that p16 suppresses breast cancer angiogenesis and metastasis, and downregulates VEGF gene expression by neutralizing the transactivation of the VEGF transcriptional factor HIF-1α. Hypoxia stimulates tumor malignant progression and induces HIF-1α. Because p16 neutralizes effect of HIF-1α and attenuates tumor metastatic progression, we intended to investigate whether p16 directly affects one or more aspects of the malignant process such as adhesion and migration of breast cancer cells. To approach this aim, MDA-MB-231 and other breast cancer cells stably transfected with Tet-on inducible p16 were used to study the p16 effect on growth, adhesion and migration of the cancer cells. We found that p16 inhibits breast cancer cell proliferation and migration, but has no apparent effect on cell adhesion. Importantly, p16 inhibits hypoxia-induced cell migration in breast cancer in parallel with its inhibition of HIF-1α transactivation activity. This study suggests that p16's ability to suppress tumor metastasis may be partially resulted from p16's inhibition on cell migration, in addition to its known functions on inhibition of cell proliferation, angiogenesis and induction of apoptosis.

Original languageEnglish (US)
Pages (from-to)126-135
Number of pages10
JournalJournal of Cancer
Volume1
Issue number1
DOIs
StatePublished - Jan 1 2010

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Cell Movement
Breast Neoplasms
Vascular Endothelial Growth Factor A
Transcriptional Activation
Cell Proliferation
Cell Migration Inhibition
Neoplasm Metastasis
Neoplasms
Cell Adhesion
Down-Regulation
Hypoxia
Apoptosis
Gene Expression
Growth

All Science Journal Classification (ASJC) codes

  • Oncology

Cite this

Inhibition of hypoxia-induced cell motility by p16 in MDA-MB-231 breast cancer cells. / Li, Liyuan; Lu, Yi.

In: Journal of Cancer, Vol. 1, No. 1, 01.01.2010, p. 126-135.

Research output: Contribution to journalArticle

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