Inhibition of succinic semialdehyde dehydrogenase by N-formylglycine

Santosh Kumar, Narayan S. Punekar

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

N-formylglycine was developed as a dead-end inhibitor of the succinic semialdehyde dehydrogenase reaction. At 4 mM, it inhibited Aspergillus niger succinic semialdehyde dehydrogenase by 40%. N-formylglycine is a reversible, complete inhibitor; the inhibition is competitive with succinic semialdehyde and uncompetitive with respect to NAD+ and the K(i) values are 4.9 and 10.4 mM respectively. Potato succinic semialdehyde dehydrogenase is also inhibited by N-formylglycine to a similar extent, the nature of the inhibition being identical to that observed with the A. niger enzyme.

Original languageEnglish (US)
Pages (from-to)369-376
Number of pages8
JournalJournal of Enzyme Inhibition
Volume13
Issue number5
DOIs
StatePublished - Jan 1 1998

Fingerprint

Succinate-Semialdehyde Dehydrogenase
Aspergillus niger
Aspergillus
Solanum tuberosum
NAD
Enzymes
N-formylglycine

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine

Cite this

Inhibition of succinic semialdehyde dehydrogenase by N-formylglycine. / Kumar, Santosh; Punekar, Narayan S.

In: Journal of Enzyme Inhibition, Vol. 13, No. 5, 01.01.1998, p. 369-376.

Research output: Contribution to journalArticle

@article{85af46de1d91482e8cdaa0337fa65256,
title = "Inhibition of succinic semialdehyde dehydrogenase by N-formylglycine",
abstract = "N-formylglycine was developed as a dead-end inhibitor of the succinic semialdehyde dehydrogenase reaction. At 4 mM, it inhibited Aspergillus niger succinic semialdehyde dehydrogenase by 40{\%}. N-formylglycine is a reversible, complete inhibitor; the inhibition is competitive with succinic semialdehyde and uncompetitive with respect to NAD+ and the K(i) values are 4.9 and 10.4 mM respectively. Potato succinic semialdehyde dehydrogenase is also inhibited by N-formylglycine to a similar extent, the nature of the inhibition being identical to that observed with the A. niger enzyme.",
author = "Santosh Kumar and Punekar, {Narayan S.}",
year = "1998",
month = "1",
day = "1",
doi = "10.3109/14756369809021482",
language = "English (US)",
volume = "13",
pages = "369--376",
journal = "Journal of Enzyme Inhibition and Medicinal Chemistry",
issn = "1475-6366",
publisher = "Informa Healthcare",
number = "5",

}

TY - JOUR

T1 - Inhibition of succinic semialdehyde dehydrogenase by N-formylglycine

AU - Kumar, Santosh

AU - Punekar, Narayan S.

PY - 1998/1/1

Y1 - 1998/1/1

N2 - N-formylglycine was developed as a dead-end inhibitor of the succinic semialdehyde dehydrogenase reaction. At 4 mM, it inhibited Aspergillus niger succinic semialdehyde dehydrogenase by 40%. N-formylglycine is a reversible, complete inhibitor; the inhibition is competitive with succinic semialdehyde and uncompetitive with respect to NAD+ and the K(i) values are 4.9 and 10.4 mM respectively. Potato succinic semialdehyde dehydrogenase is also inhibited by N-formylglycine to a similar extent, the nature of the inhibition being identical to that observed with the A. niger enzyme.

AB - N-formylglycine was developed as a dead-end inhibitor of the succinic semialdehyde dehydrogenase reaction. At 4 mM, it inhibited Aspergillus niger succinic semialdehyde dehydrogenase by 40%. N-formylglycine is a reversible, complete inhibitor; the inhibition is competitive with succinic semialdehyde and uncompetitive with respect to NAD+ and the K(i) values are 4.9 and 10.4 mM respectively. Potato succinic semialdehyde dehydrogenase is also inhibited by N-formylglycine to a similar extent, the nature of the inhibition being identical to that observed with the A. niger enzyme.

UR - http://www.scopus.com/inward/record.url?scp=0031707752&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031707752&partnerID=8YFLogxK

U2 - 10.3109/14756369809021482

DO - 10.3109/14756369809021482

M3 - Article

C2 - 9793840

AN - SCOPUS:0031707752

VL - 13

SP - 369

EP - 376

JO - Journal of Enzyme Inhibition and Medicinal Chemistry

JF - Journal of Enzyme Inhibition and Medicinal Chemistry

SN - 1475-6366

IS - 5

ER -