INHIBITORY EFFECT OF ADENOSINE AND ADENINE NUCLEOTIDES ON POTASSIUM‐EVOKED EFFLUX OF [3H]‐NORADRENALINE FROM THE RAT ISOLATED HEART: LACK OF RELATIONSHIP TO PROSTAGLANDINS

MOHAMMAD T. KHAN, Kafait Malik

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Abstract

In the isolated heart of the rat prelabelled with [3H]‐noradrenaline (NA) and perfused with Krebs solution, administration of potassium (K+ 60 μmol) increased the efflux of total radioactivity and of [3H]‐NA in the perfusate. Adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine, but not inosine, dibutyryl cyclic adenosine 3′5′‐monophosphate (db cyclic AMP) or db cyclic GMP reduced the K+‐evoked overflow of total radioactivity and of intact [3H]‐NA, in concentrations too low to cause release of prostaglandins. ATP, ADP and adenosine did not affect tyramine‐evoked overflow of tritium. Blockade of prostaglandin synthesis with indomethacin did not alter the inhibitory effect of either ATP, ADP or adenosine on K+‐induced overflow of tritium, thereby indicating that these nucleotides inhibit adrenergic transmission by a mechanism unrelated to stimulation of prostaglandin synthesis. Theophylline which increases entry of calcium (Ca2+) across the cell membrane and reduces its binding in the cell, enhanced K+‐evoked overflow of tritium and diminished the inhibitory effect of ATP, ADP and adenosine on K+‐evoked overflow of tritium from the heart, presumably by interfering with transneuronal Ca2+ metabolism. 1980 British Pharmacological Society

Original languageEnglish (US)
Pages (from-to)551-561
Number of pages11
JournalBritish Journal of Pharmacology
Volume68
Issue number3
DOIs
StatePublished - Jan 1 1980

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Adenine Nucleotides
Adenosine
Tritium
Prostaglandins
Norepinephrine
Adenosine Diphosphate
Adenosine Triphosphate
Radioactivity
Inosine
Cyclic GMP
Theophylline
Indomethacin
Adrenergic Agents
Potassium
Nucleotides
Cell Membrane
Calcium

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

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title = "INHIBITORY EFFECT OF ADENOSINE AND ADENINE NUCLEOTIDES ON POTASSIUM‐EVOKED EFFLUX OF [3H]‐NORADRENALINE FROM THE RAT ISOLATED HEART: LACK OF RELATIONSHIP TO PROSTAGLANDINS",
abstract = "In the isolated heart of the rat prelabelled with [3H]‐noradrenaline (NA) and perfused with Krebs solution, administration of potassium (K+ 60 μmol) increased the efflux of total radioactivity and of [3H]‐NA in the perfusate. Adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine, but not inosine, dibutyryl cyclic adenosine 3′5′‐monophosphate (db cyclic AMP) or db cyclic GMP reduced the K+‐evoked overflow of total radioactivity and of intact [3H]‐NA, in concentrations too low to cause release of prostaglandins. ATP, ADP and adenosine did not affect tyramine‐evoked overflow of tritium. Blockade of prostaglandin synthesis with indomethacin did not alter the inhibitory effect of either ATP, ADP or adenosine on K+‐induced overflow of tritium, thereby indicating that these nucleotides inhibit adrenergic transmission by a mechanism unrelated to stimulation of prostaglandin synthesis. Theophylline which increases entry of calcium (Ca2+) across the cell membrane and reduces its binding in the cell, enhanced K+‐evoked overflow of tritium and diminished the inhibitory effect of ATP, ADP and adenosine on K+‐evoked overflow of tritium from the heart, presumably by interfering with transneuronal Ca2+ metabolism. 1980 British Pharmacological Society",
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