Insulin-independent, MAPK-dependent stimulation of NKCC activity in skeletal muscle

Jennifer A. Wong, Aidar R. Gosmanov, Edward G. Schneider, Donald Thomason

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Na+-K+-Cl- cotransporter (NKCC) activity in quiescent skeletal muscle is modest. However, ex vivo stimulation of muscle for as little as 18 contractions (1 min, 0.3 Hz) dramatically increased the activity of the cotransporter, measured as the bumetanide-sensitive 86Rb influx, in both soleus and plantaris muscles. This activation of cotransporter activity remained relatively constant for up to 10-Hz stimulation for 1 min, falling off at higher frequencies (30-Hz stimulation for 1 min). Similarly, stimulation of skeletal muscle with adrenergic receptor agonists phenylephrine, isoproterenol, or epinephrine produced a dramatic stimulation of NKCC activity. It did not appear that stimulation of NKCC activity was a reflection of increased Na+-K+-ATPase activity because insulin treatment did not stimulate NKCC activity, despite insulin's well-known stimulation of Na+-K+-ATPase activity. Stimulation of NKCC activity could be blocked by pretreatment with inhibitors of mitogen-activated protein kinase (MAPK) kinase 1/2 (MEK1/2) activity, indicating that activation of the extracellular signal-regulated kinase 1/2 (ERK1/2) MAPKs may be required. These data indicate a regulated NKCC activity in skeletal muscle that may provide a significant pathway for potassium transport into skeletal muscle fibers.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume281
Issue number2 50-2
StatePublished - Aug 29 2001

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Sodium-Potassium-Chloride Symporters
Mitogen-Activated Protein Kinases
Skeletal Muscle
Insulin
MAP Kinase Kinase 2
MAP Kinase Kinase 1
Bumetanide
Adrenergic Agonists
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Skeletal Muscle Fibers
Phenylephrine
Isoproterenol
Epinephrine
Potassium
Muscles

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Insulin-independent, MAPK-dependent stimulation of NKCC activity in skeletal muscle. / Wong, Jennifer A.; Gosmanov, Aidar R.; Schneider, Edward G.; Thomason, Donald.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 281, No. 2 50-2, 29.08.2001.

Research output: Contribution to journalArticle

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