Insulin induces hepatic expression of apobec-1, the catalytic subunit of the apolipoprotein B mRNA editing complex in rats

M. A. Von Wronaki, L. Cagen, H. G. Wilcox, Rajendra Raghow, F. E. Thorngate, M. Heimberg, Marshall Elam

Research output: Contribution to journalArticle

Abstract

Apolipoprotein (apo) B is the major protein component of very low density lipoprotein (VLDL) and low density lipoprotein (LDL). Two forms of apoB are synthesized from a single gene: a full length form, apoBlOO, and apoB48, corresponding to the amino-terminal 48% of apoBlOO. ApoB48 arises through an editing mechanism involving apobec-1 whereby a cytidine residue near the middle of the apoB mRNA is deaminated to produce uridine, creating a new stop codon. We showed previously that chronic insulin treatment of rat hepatocytes increases the fraction of edited apoB mRNA from about 50% to as much as 90% (P.N.A.S. 91:5392-5396). We have used a quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay to measure the effect of insulin treatment on levels of apobec-1 message. Increased editing of apoB mRNA following insulin treatment was accompanied by elevated apobec-1 mRNA. This demonstrates that insulin increases apoB mRNA editing, at least in part, by transcriptional regulation of the catalytic subunit of the editing complex, apobec-1.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996

Fingerprint

apolipoprotein B
Apolipoproteins B
protein subunits
Rats
Catalytic Domain
insulin
Insulin
Messenger RNA
liver
Liver
rats
Apolipoprotein B-48
cytidine
uridine
stop codon
very low density lipoprotein
low density lipoprotein
hepatocytes
Cytidine
quantitative polymerase chain reaction

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Insulin induces hepatic expression of apobec-1, the catalytic subunit of the apolipoprotein B mRNA editing complex in rats. / Von Wronaki, M. A.; Cagen, L.; Wilcox, H. G.; Raghow, Rajendra; Thorngate, F. E.; Heimberg, M.; Elam, Marshall.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

Von Wronaki, M. A. ; Cagen, L. ; Wilcox, H. G. ; Raghow, Rajendra ; Thorngate, F. E. ; Heimberg, M. ; Elam, Marshall. / Insulin induces hepatic expression of apobec-1, the catalytic subunit of the apolipoprotein B mRNA editing complex in rats. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
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AU - Thorngate, F. E.

AU - Heimberg, M.

AU - Elam, Marshall

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