Integrin α6/β4 complex is located in hemidesmosomes, suggesting a major role in epidermal cell-basement membrane adhesion

A. Sonnenberg, J. Calafat, H. Janssen, H. Daams, L. M.H. Van Der Raaij-Helmer, R. Falconi, Stephen Kennel, J. D. Aplin, J. Baker, M. Loizidou, D. Garrod

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Abstract

The α6/β4 complex is a member of the integrin family of adhesion receptors. It is found on a variety of epithelial cell types, but is most strongly expressed on stratified squamous epithelia. Fluorescent antibody staining of human epidermis suggests that the β4 subunit is strongly localized to the basal region showing a similar distribution to that of the 230-kD bullous pemphigoid antigen. The α6 subunit is also strongly localized to the basal region but in addition is present over the entire surfaces of basal cells and some cells in the immediate suprabasal region. By contrast staining for β1, α2, and α3 subunits was very weak basally, but strong on all other surfaces of basal epidermal cells. These results suggest that different integrin complexes play differing roles in cell-cell and cell-matrix adhesion in the epidermis. Immunoelectron microscopy showed that the α6/β4 complex at the basal epidermal surface is strongly localized to hemidesmosomes. This result provides the first well-characterized monoclonal antibody markers for hemidesmosomes and suggests that the α6β4 complex plays a major role in epidermal cell-basement membrane adhesion. We suggest that the cytoplasmic domains of these transmembrane glycoproteins may contribute to the structure of hemidesmosomal plaques. Immunoultrastructural localization of the BP antigen suggests that it may be involved in bridging between hemidesmosomal plaques and keratin intermediate filaments of the cytoskeleton.

Original languageEnglish (US)
Pages (from-to)907-917
Number of pages11
JournalJournal of Cell Biology
Volume113
Issue number4
DOIs
StatePublished - Jan 1 1991

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Hemidesmosomes
Basement Membrane
Integrins
Cell Membrane
Epidermis
Cell-Matrix Junctions
Staining and Labeling
Antigens
Bullous Pemphigoid
Intermediate Filaments
Immunoelectron Microscopy
Keratins
Cytoskeleton
Glycoproteins
Epithelium
Epithelial Cells
Monoclonal Antibodies
Antibodies

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Sonnenberg, A., Calafat, J., Janssen, H., Daams, H., Van Der Raaij-Helmer, L. M. H., Falconi, R., ... Garrod, D. (1991). Integrin α6/β4 complex is located in hemidesmosomes, suggesting a major role in epidermal cell-basement membrane adhesion. Journal of Cell Biology, 113(4), 907-917. https://doi.org/10.1083/jcb.113.4.907

Integrin α6/β4 complex is located in hemidesmosomes, suggesting a major role in epidermal cell-basement membrane adhesion. / Sonnenberg, A.; Calafat, J.; Janssen, H.; Daams, H.; Van Der Raaij-Helmer, L. M.H.; Falconi, R.; Kennel, Stephen; Aplin, J. D.; Baker, J.; Loizidou, M.; Garrod, D.

In: Journal of Cell Biology, Vol. 113, No. 4, 01.01.1991, p. 907-917.

Research output: Contribution to journalArticle

Sonnenberg, A, Calafat, J, Janssen, H, Daams, H, Van Der Raaij-Helmer, LMH, Falconi, R, Kennel, S, Aplin, JD, Baker, J, Loizidou, M & Garrod, D 1991, 'Integrin α6/β4 complex is located in hemidesmosomes, suggesting a major role in epidermal cell-basement membrane adhesion', Journal of Cell Biology, vol. 113, no. 4, pp. 907-917. https://doi.org/10.1083/jcb.113.4.907
Sonnenberg, A. ; Calafat, J. ; Janssen, H. ; Daams, H. ; Van Der Raaij-Helmer, L. M.H. ; Falconi, R. ; Kennel, Stephen ; Aplin, J. D. ; Baker, J. ; Loizidou, M. ; Garrod, D. / Integrin α6/β4 complex is located in hemidesmosomes, suggesting a major role in epidermal cell-basement membrane adhesion. In: Journal of Cell Biology. 1991 ; Vol. 113, No. 4. pp. 907-917.
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abstract = "The α6/β4 complex is a member of the integrin family of adhesion receptors. It is found on a variety of epithelial cell types, but is most strongly expressed on stratified squamous epithelia. Fluorescent antibody staining of human epidermis suggests that the β4 subunit is strongly localized to the basal region showing a similar distribution to that of the 230-kD bullous pemphigoid antigen. The α6 subunit is also strongly localized to the basal region but in addition is present over the entire surfaces of basal cells and some cells in the immediate suprabasal region. By contrast staining for β1, α2, and α3 subunits was very weak basally, but strong on all other surfaces of basal epidermal cells. These results suggest that different integrin complexes play differing roles in cell-cell and cell-matrix adhesion in the epidermis. Immunoelectron microscopy showed that the α6/β4 complex at the basal epidermal surface is strongly localized to hemidesmosomes. This result provides the first well-characterized monoclonal antibody markers for hemidesmosomes and suggests that the α6β4 complex plays a major role in epidermal cell-basement membrane adhesion. We suggest that the cytoplasmic domains of these transmembrane glycoproteins may contribute to the structure of hemidesmosomal plaques. Immunoultrastructural localization of the BP antigen suggests that it may be involved in bridging between hemidesmosomal plaques and keratin intermediate filaments of the cytoskeleton.",
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AU - Van Der Raaij-Helmer, L. M.H.

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