Interaction between duodenase and α1-proteinase inhibitor

I. P. Gladysheva, N. A. Popykina, T. S. Zamolodchikova, N. I. Larionova

Research output: Contribution to journalArticle

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Abstract

The interaction between duodenase, a newly recognized serine proteinase belonging to the small group of Janus-faced proteinases, and α1-proteinase inhibitor (α1-PI) from human serum was investigated. The stoichiometry of the inhibition was 1.2 mol/mol. The presence of a stable enzyme-inhibitor complex was shown by SDS-PAGE. The mechanism of interaction between duodenase and α1-PI was shown to be of the suicide type. The equilibrium and inhibition constants are 13 ± 3 nM and (1.9 ± 0.3)·105 M-1·sec-1, respectively. Based on the association rate constant of the enzyme-inhibitor complex and localization of duodenase and α1-PI in identical compartments, α1-PI is suggested to be a duodenase inhibitor in vivo.

Original languageEnglish (US)
Pages (from-to)682-687
Number of pages6
JournalBiochemistry (Moscow)
Volume66
Issue number6
DOIs
StatePublished - Jun 1 2001

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Peptide Hydrolases
Enzyme Inhibitors
Serine Proteases
Stoichiometry
Suicide
Polyacrylamide Gel Electrophoresis
Rate constants
duodenase
Association reactions
Serum
Inhibition (Psychology)

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Gladysheva, I. P., Popykina, N. A., Zamolodchikova, T. S., & Larionova, N. I. (2001). Interaction between duodenase and α1-proteinase inhibitor. Biochemistry (Moscow), 66(6), 682-687. https://doi.org/10.1023/A:1010267500296

Interaction between duodenase and α1-proteinase inhibitor. / Gladysheva, I. P.; Popykina, N. A.; Zamolodchikova, T. S.; Larionova, N. I.

In: Biochemistry (Moscow), Vol. 66, No. 6, 01.06.2001, p. 682-687.

Research output: Contribution to journalArticle

Gladysheva, IP, Popykina, NA, Zamolodchikova, TS & Larionova, NI 2001, 'Interaction between duodenase and α1-proteinase inhibitor', Biochemistry (Moscow), vol. 66, no. 6, pp. 682-687. https://doi.org/10.1023/A:1010267500296
Gladysheva IP, Popykina NA, Zamolodchikova TS, Larionova NI. Interaction between duodenase and α1-proteinase inhibitor. Biochemistry (Moscow). 2001 Jun 1;66(6):682-687. https://doi.org/10.1023/A:1010267500296
Gladysheva, I. P. ; Popykina, N. A. ; Zamolodchikova, T. S. ; Larionova, N. I. / Interaction between duodenase and α1-proteinase inhibitor. In: Biochemistry (Moscow). 2001 ; Vol. 66, No. 6. pp. 682-687.
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