Interaction of coxsackievirus B3 with the full length coxsackievirus-adenovirus receptor

Yongning He, Paul R. Chipman, Jason Howitt, Carol M. Bator, Michael Whitt, Timothy S. Baker, Richard J. Kuhn, Carl W. Anderson, Paul Freimuth, Michael G. Rossmann

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

Group B coxsackieviruses (CVB) utilize the coxsackievirus-adenovirus receptor (CAR) to recognize host cells. CAR is a membrane protein with two Ig-like extracellular domains (D1 and D2), a transmembrane domain and a cytoplasmic domain. The three-dimensional structure of coxsackievirus B3 (CVB3) in complex with full length human CAR and also with the D1D2 fragment of CAR were determined to ∼22 Å resolution using cryo-electron microscopy (cryo-EM). Pairs of transmembrane domains of CAR associate with each other in a detergent cloud that mimics a cellular plasma membrane. This is the first view of a virus-receptor interaction at this resolution that includes the transmembrane and cytoplasmic portion of the receptor. CAR binds with the distal end of domain D1 in the canyon of CVB3, similar to how other receptor molecules bind to entero- and rhinoviruses. The previously described interface of CAR with the adenovirus knob protein utilizes a side surface of D1.

Original languageEnglish (US)
Pages (from-to)874-878
Number of pages5
JournalNature Structural Biology
Volume8
Issue number10
DOIs
StatePublished - Oct 14 2001

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Enterovirus
Knobs
Virus Receptors
Cell membranes
Cytoplasmic and Nuclear Receptors
adenovirus receptor
Detergents
Cryoelectron Microscopy
Human Enterovirus B
Electron microscopy
Human Adenoviruses
Rhinovirus
Membrane Proteins
Adenoviridae
Molecules
Cell Membrane
Proteins

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Genetics

Cite this

He, Y., Chipman, P. R., Howitt, J., Bator, C. M., Whitt, M., Baker, T. S., ... Rossmann, M. G. (2001). Interaction of coxsackievirus B3 with the full length coxsackievirus-adenovirus receptor. Nature Structural Biology, 8(10), 874-878. https://doi.org/10.1038/nsb1001-874

Interaction of coxsackievirus B3 with the full length coxsackievirus-adenovirus receptor. / He, Yongning; Chipman, Paul R.; Howitt, Jason; Bator, Carol M.; Whitt, Michael; Baker, Timothy S.; Kuhn, Richard J.; Anderson, Carl W.; Freimuth, Paul; Rossmann, Michael G.

In: Nature Structural Biology, Vol. 8, No. 10, 14.10.2001, p. 874-878.

Research output: Contribution to journalArticle

He, Y, Chipman, PR, Howitt, J, Bator, CM, Whitt, M, Baker, TS, Kuhn, RJ, Anderson, CW, Freimuth, P & Rossmann, MG 2001, 'Interaction of coxsackievirus B3 with the full length coxsackievirus-adenovirus receptor', Nature Structural Biology, vol. 8, no. 10, pp. 874-878. https://doi.org/10.1038/nsb1001-874
He, Yongning ; Chipman, Paul R. ; Howitt, Jason ; Bator, Carol M. ; Whitt, Michael ; Baker, Timothy S. ; Kuhn, Richard J. ; Anderson, Carl W. ; Freimuth, Paul ; Rossmann, Michael G. / Interaction of coxsackievirus B3 with the full length coxsackievirus-adenovirus receptor. In: Nature Structural Biology. 2001 ; Vol. 8, No. 10. pp. 874-878.
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